Figure 5From: sigE facilitates the adaptation of Bordetella bronchiseptica to stress conditions and lethal infection in immunocompromised miceRB50Δ sigE is less cytotoxic to macrophages than RB50. RAW 264.7 cells were incubated at an MOI of 10 with medium containing RB50 pEV, RB50ΔsigE pEV, RB50ΔsigE pSigE, TTSS-deficient RB50 strain WD3, or medium alone for 4 hours in the presence of 1 mM IPTG to induce expression of sigE from the pLac promoter of pSigE. The average percent cytotoxicity of four wells in four separate experiments as measured by (LDH release from a well/LDH release from the positive control well) x100 ± SE is shown. The differences in percent cytotoxicity between RB50ΔsigE pEV and either RB50 pEV or RB50ΔsigE pSigE are statistically significant (** indicates P value < 0.01), while the cytotoxicities of RB50 pEV and RB50ΔsigE pSigE are not significantly different.Back to article page