The H. pylori rocF- mutant induces more IL-8 and MIP-1 β in AGS cells than wild type H. pylori, as determined by Bioplex. Supernatants from H. pylori infected-AGS cells were collected and used to determine the concentration of IL-8 and MIP-1β (pg/ml) A. Levels of IL-8; one-way ANOVA p < 0.0001; *p = 0.0001 (rocF- vs NS); #p = 0.0249 (rocF- vs WT); **p = 0.044 (rocF- vs rocF+); B. Levels of MIP-1B; one-way ANOVA p < 0.0001; *p < 0.0001 (rocF- vs NS); #p < 0.0001 (rocF- vs WT); p = 0.0001 (rocF- vs rocF+). Values in both Figures represent the average signal ± SEM of four independent replicates.