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Table 3 Induced proteins (% volume intensity) during HCl (pH 5.2) and acetic acid (pH 5.7) exposure in C. jejuni NCTC 11168, C. jejuni 305 and C. jejuni 327 at 37°C in chemically defined broth

From: Acid stress response and protein induction in Campylobacter jejuni isolates with different acid tolerance

     Campylobacter jejunistrains3
Protein/(NCBInr 1 ) Mw (kDa) Score 2   NCTC 11168 305 327
Dps (NP282665) 17.4 222 Vol%
p19 (CAA73983) 17.0 255 Vol%
AhpC (NP281525) 22.0 668 Vol%
SodB (NP281379) 25.0 241 Vol%
TrxB (NP281357) 33.5 204 Vol%
Cj0706 (NP281878) 28.0 431 Vol%
MogA (YP_178829) 20.3 318 Vol%
  C HCl Ac C HCl Ac C HCl Ac
  1. Dps: Bacterioferritin, p19: 19 kDa periplasmic protein, AhpC: Alkyl hydroperoxide reductase, SodB: Superoxide dismutase (Fe), TrxB: Thioredoxin-disulfide reductase, Cj0706: hypothetical protein, MogA: Molybdenum cofactor biosynthesis protein.
  2. Columns: light grey: control (C), dark grey: HCl stressed cells (HCl), white: Acetic acid stressed cells (Ac).
  3. 1 Identification was based on Mascot MS/MS Ion Search using sequence data from the database NCBInr.
  4. 2 Mowse Score (Score).
  5. 3 The intensity of the induced proteins was estimated by Image MasterTM 2D Platinum and % volume intensity was calculated.
  6. The intensity of the protein spots was analyzed using the Image MasterTM 2D Platinum (version 5.0, Amersham Biosciences, Melanie). Three biological independent replicates was performed and % volume intensity was calculated as: % volume intensity control (protein x) = volume intensity /(volume intensity control + volume intensity HCl + volume intensity acetic acid).
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BMC Microbiology

ISSN: 1471-2180

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