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Table 1 Components of modified chemically defined broth (CDB) for Campylobacter jejuni

From: Acid stress response and protein induction in Campylobacter jejuni isolates with different acid tolerance

Components Stock solution (mg/ml) Vol stock solution (ml) for 1 liter Final conc (mmol/l) of 1xCDB
Buffer solution (10X)   100.0  
K2HPO4 34.8   20.0
KH2PO4 27.2   20.0
Salt solution (20X)   50.0  
NaCl 116.0   100.00
K2SO4 20.0   5.74
MgCl2, 6 H2O 8.2   2.02
NH4Cl 4.4   4.11
EDTA 0.074   0.01
Amino acid mix 1 (100X)   10.0  
L-Arginine HCl 15.0   0.71
L-serine 5.0   0.48
L-leucine 9.0   0.69
L-isoleucine 3.0   0.23
L-valine 6.0   0.51
L-proline 5.0   0.43
L-phenylalanine 5.0   0.30
L-alanine 10.0   1.12
L-histidine 5.0   0.32
L-threonine 5.0   0.42
L-lysine 5.0   0.30
L-glycine 2.5   0.33
L-trypthophan 8.0   0.39
Amino acid mix 2 (10X)   100.0  
L-aspartate 5.0   3.76
L-glutamate 13.0   8.83
Individual amino acids    
L-cysteine/HCl † 17.5 3.0 0.35
L-cysteine † 12.0 3.5 0.15
L-methionine 14.9 1.0 0.10
Vitamin mix (50X)   0.2  
NAD 10.0   0.0030
Thiamine HCl (Vitamine B1) 10.0   0.0060
Calcium pantothenate (Vitamine B5) 10.0   0.0040
Individual components    
Oxaloacetate, 2 H2O (10X) 2.5 100.0  
NaHCO3 (2000×) 84.0 0.5 1.49
Biotin (Vitamine H) (500X) 0.25 2.0 0.50
Fe(NO3)3, 9 H2O (1000X) 4.0 1.0 0.0021
CaCl2, 2 H2O (1000X) 37.0   0.01
    0.25
Total (ml)   372.2  
MilliQ water (ml) *   627.8  
  1. The broth was prepared as described in the text.
  2. *: To make portions of 500 ml 2 × CDB without methionine and cysteine, only 127.8 ml of MilliQ water was added. The batches were sterilized by filtration, aliquoted, and stored at −20°C.
  3. †: Cysteine was prepared freshly and dissolved in 1 M HCl prior to each experiment.