Table 1 Components of modified chemically defined broth (CDB) for Campylobacter jejuni
Components | Stock solution (mg/ml) | Vol stock solution (ml) for 1 liter | Final conc (mmol/l) of 1xCDB |
|---|---|---|---|
Buffer solution (10X) | 100.0 | ||
K2HPO4 | 34.8 | 20.0 | |
KH2PO4 | 27.2 | 20.0 | |
Salt solution (20X) | 50.0 | ||
NaCl | 116.0 | 100.00 | |
K2SO4 | 20.0 | 5.74 | |
MgCl2, 6 H2O | 8.2 | 2.02 | |
NH4Cl | 4.4 | 4.11 | |
EDTA | 0.074 | 0.01 | |
Amino acid mix 1 (100X) | 10.0 | ||
L-Arginine HCl | 15.0 | 0.71 | |
L-serine | 5.0 | 0.48 | |
L-leucine | 9.0 | 0.69 | |
L-isoleucine | 3.0 | 0.23 | |
L-valine | 6.0 | 0.51 | |
L-proline | 5.0 | 0.43 | |
L-phenylalanine | 5.0 | 0.30 | |
L-alanine | 10.0 | 1.12 | |
L-histidine | 5.0 | 0.32 | |
L-threonine | 5.0 | 0.42 | |
L-lysine | 5.0 | 0.30 | |
L-glycine | 2.5 | 0.33 | |
L-trypthophan | 8.0 | 0.39 | |
Amino acid mix 2 (10X) | 100.0 | ||
L-aspartate | 5.0 | 3.76 | |
L-glutamate | 13.0 | 8.83 | |
Individual amino acids | |||
L-cysteine/HCl † | 17.5 | 3.0 | 0.35 |
L-cysteine † | 12.0 | 3.5 | 0.15 |
L-methionine | 14.9 | 1.0 | 0.10 |
Vitamin mix (50X) | 0.2 | ||
NAD | 10.0 | 0.0030 | |
Thiamine HCl (Vitamine B1) | 10.0 | 0.0060 | |
Calcium pantothenate (Vitamine B5) | 10.0 | 0.0040 | |
Individual components | |||
Oxaloacetate, 2 H2O (10X) | 2.5 | 100.0 | |
NaHCO3 (2000×) | 84.0 | 0.5 | 1.49 |
Biotin (Vitamine H) (500X) | 0.25 | 2.0 | 0.50 |
Fe(NO3)3, 9 H2O (1000X) | 4.0 | 1.0 | 0.0021 |
CaCl2, 2 H2O (1000X) | 37.0 | 0.01 | |
0.25 | |||
Total (ml) | 372.2 | ||
MilliQ water (ml) * | 627.8 |