Table 2 Primer
Gene | Primer name | Sequence 5’-3’ | Annealing temp | Reference |
|---|---|---|---|---|
cj0178 | cj0178-F01 | TGTAGGCGGGGGTGGCAAGA | 54.0°C | this study |
cj0178-R01 | ACGACCGCGAGCAGAATTGC | |||
cj0755/cfrA | cj0755/cfrA-F01 | ATGGCCGCGAAGTCGTAGGG | 54.0°C | this study |
cj0755/cfrA-R01 | AGCGATCTATTTGCCACTCGCCT | |||
cj1321 | CjNCTC11168-1321_f | AAAATGTCATCATCATAGGAGCG | 60.0°C | [6] |
CjNCTC11168-1321_r | TCTAAGTTTACGCAAGGCAACA | |||
cj1322 | CjNCTC11168-1322_f | GACTTTGGTTTAATGGGTAAGCA | 59.6°C | [6] |
CjNCTC11168-1322_r | TTCCGGCGTTAAAATTAGAAAA | |||
cj1323 | CjNCTC11168-1323_f | AGAACGATTTACCCCATTGAAA | 59.7°C | [6] |
CjNCTC11168-1323_r | ATTTGCTAAAGCTCCTCGATTG | |||
cj1324 | CjNCTC11168-1324_f | TGCCGTAAGTGGAGGTAAAGAT | 60.0°C | [6] |
CjNCTC11168-1324_r | TCTGCACACATTGTTCTATCCC | |||
cj1325 | CjNCTC11168-1325_f | ACGGATTACTTTTTCCAGATGGT | 60.0°C | [6] |
CjNCTC11168-1325_r | TTTGCTTTGAAAATACGCTGAA | |||
cj1326 | CjNCTC11168-1326_f | TACATTTCATCGATAAAGCCGA | 59.7°C | [6] |
CjNCTC11168-1326_r | AAATATAATGGTGTGCCGATCC | |||
fucP | cj0486FWD | GATAGAGCATTAAATTGGGATG | 52.0°C | [8] |
cj0486REV | CCTATAAAGCCATACCAAGCC | |||
rpoC | GAACTTGCTATTGCTGAGCC | |||
rpsL | ACCCTAGTGCAAACTCCCCT | |||
ceuE | ceuE-81176F01 | GATAGAGTCGCAGGCGTTCC | 60°C | this study |
ceuE405F | GATAAAGTCGTTGGCGTTCC | [7] | ||
ceuE405R | GCGAGATTGGAGGACCAAAGG | |||
pldA | pldA-81178F01 | AAACTTATGCGTTTTT | 45°C | this study |
pldA-84fwd | AAGCTTATGCGTTTTT | [7] | ||
pld-981rev | TATAAGGCTTTCTCCA | |||
cstII | orf7ab | ACTACACTTTAAAACATTTAATCC AAAATCA | 56°C | [14] |
orf7ab | CCATAAGCCTCACTAGAAGGTATGAGTATA | |||
cstIII | orf7c | TTGAAGATAGATATTTTGTGGGTAAA | 56°C | [14] |
orf7c | CTTTAAGTAGTGTTTTATGTCACTTGG |