Table 2 Oligonucleotide primers used in this study
Primer name | Sequence (5'-3')a | reference |
|---|---|---|
esxAdeletion | ||
oBS43 | GGGGACAAGTTTTGTACAAAAAAGCAGGCTacgtttatcaaagacatacc | This study |
oBS44 | gggggtaccaactagaaacctcctgaata | This study |
oBS45 | gggggtaccgcattctgaaattggcaaag | This study |
oBS46 | GGGGACCACTTTGTACAAGAAAGCTGGGTttatccatcgctgtattgtg | This study |
DIG probes | ||
Nwmn0219-DIG-f | tccagaggaaatcagagcaaa | [10] |
Nwmn0219-DIG-r | cttgttcttgaacggcatca | [10] |
oSTM29 (spoVGf) | gcgtcgacttattgcaaatgtattacatcgc | [9] |
oSTM43 (spoVGr) | gcggagctccactcgtttccattacattagatg | [8] |
SasarAf | agggaggttttaaacatggc | [35] |
SasarAf | ctcgactcaataatgattcg | [35] |
RNAIII+ | gtgatggaaaatagttgatgag | [3] |
RNAIII- | gtgaatttgttcactgtgtcg | [3] |
arlRSprobe+ | tcgtatcacatacccaacgc | [36] |
arlRSprobe- | gagtatgatggacaagacgg | [36] |
SAasp23+ | atgactgtagataacaataaagc | [37] |
SAasp23- | ttgtaaaccttgtctttcttgg | [37] |
plasmid construction | ||
Pnwmn0219F | tgcggatccgatcacgttgatttgcgtgt | This study |
Pnwmn0219R-xho | tgcctcgagctagaaacctcctgaatattttaag | This study |
yab-prom-bam-f | gcgggatcctgctaatattttaaatttacc | This study |
yab-prom-xho-r | gcgctcgagtactaaaactccttttatgaaaac | This study |
Pnwmn0219F-hind | tgcaagcttgatcacgttgatttgcgtgt | This study |
Pnwmn0219R | tgcccatggctagaaacctcctgaatattttaag | This study |
pSP-Luc XhoI | accggcctcgagatcgatgatatcgaa | This study |
oBS49 | tagttttttaagtatttttagtttttttta | This study |
oBS51 | attcaatatatttatttaaaaaaaactaaaaa | This study |
oBS53 | aggtaccttgagtaaggagcactttttcaa | This study |
oBS54 | aggtaccattcatttttgtaatataaatgtgtatac | This study |
primer extension | This study | |
pe_esxA_1 | BIOTIN-ccataactagaaacctcctg | This study |
pe_esxA_2 | BIOTIN-tgatttcctctggactcatc | This study |
esxA_term-r | tgcggtaccatgcttatttcctttcagttg | This study |