Figure 3

Cluster dendrogram of Sma I pulsed-field gel electrophoresis patterns of the five positive and confirmed S. lugdunensis isolates. Colonies of each isolate were lysed using lysostaphin and DNA was subsequently digested with SmaI. Pulsed-field gel electrophoresis (PFGE) was performed using the CHEF-DR III system on a 1% agarose in 0.5 X TBE buffer for a run time of 18 h, with a voltage of 6 V/cm, pulses ramped from 4.0 to 40.0 s, at an angle of 120°. Gels were stained with ethidium bromide and photographed using a Gel Doc 2000 before band patterns were analyzed with BioNumerics version 3.0.