Figure 3From: Pathogenic Mycobacterium bovis strains differ in their ability to modulate the proinflammatory activation phenotype of macrophagesThe activation profiles of macrophages infected with pathogenic mycobacteria. BMDM were infected with the studied mycobacterial strains at a MOI of 5:1, washed and incubated for an additional 48 h. The cells left untreated and cells stimulated with LPS for 48 h were used as a negative and positive controls of proinflammatory activation, respectively. To evaluate markers of M1-type activation (A), the culture supernatants of infected cultures were harvested and tested for TNF-α, IL-6, MCP-1, MIP-2 and IL-12 by Bioplex test, and for NO production by Griess reaction. Assays were completed with duplicate samples, and results are expressed as a mean of three independent experiments. Lines over bars indicate the isolates for which the induced cytokine production differed significantly from that induced by H37Rv (*p < 0.05; ***p < 0.001). To evaluate markers of M2-type activation, secretion of IL-10 was quantified by Bioplex assay (B), and expression of Arginase 1 and MR/CD206 in the adhered cells was tested by Western blotting (C). Lower panel, quantification of the protein levels by densitometric analysis of immunoreactive bands.Back to article page