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Figure 8 | BMC Microbiology

Figure 8

From: Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

Figure 8

Western blotting of glycoprotein fractions with anti-SCARB2 antibody. Lane 1: RD cell membrane extracts; Lane 2: MAA/SNA lectin affinity chromatography purified sialylated glycoproteins; Lane 3: desialylated glycoproteins; Lane 4: desialylated glycoproteins immunoprecipitated with EV71 MP4. All of the purified proteins were subjected to western blotting and stained by anti-SCARB2 antibody. SCARB2 could be observed in all of the fractions. Band in lane 3 was slightly shifted down after neuraminidase treatment. But, owing to non-reducing condition, band in lane 4 was slightly shifted up compared to band in lane 3.

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