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Figure 3 | BMC Microbiology

Figure 3

From: Expression of sialic acids and other nonulosonic acids in Leptospira

Figure 3

‚ÄČLeptospira interrogans‚ÄČ genome strain expresses sialic acid (Neu5Ac). HPLC analysis demonstrates peaks consistent with Kdo and Neu5Ac in Leptospira interrogans str. L1-130. Confirmation of the L1-130 Neu5Ac peak assignment was performed by parallel derivatization and LCMS analysis of Neu5Ac (Sigma). The structure of DMB-derivatized Neu5Ac has a protonated exact mass (m+H) of 426.1. The dehydrated and sodium adduct have expected m+H values of 408.1 and 448.1 respectively. This experiment was performed twice with similar results.

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