Figure 3From: Expression of sialic acids and other nonulosonic acids in Leptospira Leptospira interrogans  genome strain expresses sialic acid (Neu5Ac). HPLC analysis demonstrates peaks consistent with Kdo and Neu5Ac in Leptospira interrogans str. L1-130. Confirmation of the L1-130 Neu5Ac peak assignment was performed by parallel derivatization and LCMS analysis of Neu5Ac (Sigma). The structure of DMB-derivatized Neu5Ac has a protonated exact mass (m+H) of 426.1. The dehydrated and sodium adduct have expected m+H values of 408.1 and 448.1 respectively. This experiment was performed twice with similar results.Back to article page