Storage conditions of intestinal microbiota matter in metagenomic analysis

BMC Microbiology

Table 1 Percentage of DNA compared to the frozen samples

	% degraded DNA				n = 4
	#1	#2	#3	#4	p value when compared to frozen samples
F	12	28	10	9
UF1h	12	24	23	34	< 0.01
UF3h	25	39	31	34	< 0.001
RT3h	17	16	12	15	0.9270
RT24h	84	44	13	15	< 0.001
RT2w	48	38	26	40	< 0.001

Statistical analysis was performed using Poisson regression model; p value < 0.05 is considered significant; #1, #2, #3, #4 correspond to subjects 1, 2, 3, 4; F = frozen; UF1h = unfrozen during 1 h; UF3h = unfrozen during 3 h; RT = room temperature; 2w = 2 weeks.

ISSN: 1471-2180