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Table 1 Polypeptide spots that showed at least a 10-fold increase in relative abundance in B31 or N40D10/E9 on 2D protein gel

From: Comparative molecular analyses of Borrelia burgdorferi sensu stricto strains B31 and N40D10/E9 and determination of their pathogenicity

Spot # pI MW (kDa) Relative abundance in B31, and N40 (%) Fold change B31 vs N40 Identification MALDI-MS analyses (SwissProt or NCBI accession #) Spot # pI MW (kDa) Relative abundance in B31, and N40 (%) Fold change N40 vs B31 Identification MALDI-MS analyses (SwissProt or NCBI accession #)
33 6.2 88.96 0.036, 0.003 11.2   136 5.6 64.58 0.002, 0.029 14.7  
110 5.1 63.92 0.050, 0.003 15.1   208 5.8 53.07 0.015, 0.340 22.7  
127 5.3 65.24 0.037, 0.003 11.5   231 6.9 52.81 0.019, 0.226 11.8  
211 6.1 55,65 0.875, 0.048 18.0   272 6.2 46.29 0.000, 0.054 685.4 *Flagellin (GI:120230), Basic membrane protein A (GI:3913169)
225 6.1 57.07 0.193, 0.005 35.3   293 6.0 43.53 0.000, 0.170 698.2 *Flagellin (GI:120230)
325 5.6 38.32 0.114, 0.010 11.3   311 6.0 39.99 0.005, 0.165 30.6  
403 5.4 31.03 0.071, 0.002 29.1   347 6.0 35.06 0.003, 0.185 59.8  
404 5.4 31.00 0.404, 0.003 124.1 OspD (GI:495462) 348 5.6 34.95 0.007, 0.258 36.3  
405 5.5 28.78 1.006, 0.031 32.7   349 6.0 34.36 0.003, 0.095 32.4  
458 5.7 26.07 0.051, 0.003 15.2   352 6.5 34.25 0.003, 0.034 12.0  
463 6.5 25.58 0.107, 0.007 15.4   354 5.9 34.62 0.004, 0.207 47.9  
465 6.3 25.49 0.077, 0.006 13.2   381 5.6 29.61 0.003, 0.032 10.4  
491 7.0 22.69 0.356, 0.012 29.8   406 6.2 28.54 0.006, 0.081 14.0  
494 6.5 23.16 1.400, 0.062 22.8   418 6.3 27.97 0.089, 1.927 21.6  
495 6.7 23.13 6.875, 0.025 278.1 Outer surface protein C (GI:3914248) 452 6.6 26.33 0.006, 0.246 42.5 30S ribosomal protein S4 (B7J2H5)
Phosphoglycolate phosphatase (GI:226320487), and hypothetical
(GI:226315606)
497 6.3 22.87 0.262, 0.022 12.1   479 6.4 24.51 0.060, 0.648 10.7  
519 7.1 20.08 0.734, 0.027 26.8   501 6.5 22.47 0.030, 1.956 64.6 Same as 505
525 6.4 21.03 0.234, 0.008 30.9 Neutrophil activating protein (GI:15595035) 505 6.3 22.33 0.017, 0.570 34.0 OspC (GI: 226246807) Neutrophil activating protein (GI:15595035)
528 6.2 20.95 0.068, 0.004 15.9   517 6.2 21.41 0.002, 0.095 54.4  
541 6.4 20.31 0.097, 0.005 20.8   543 5.6 19.67 0.006, 0.072 11.9  
559 5.6 17.70 0.137, 0.008 17.2   551 6.2 19.51 0.075, 0.762 10.2  
581 4.9 11.91 2.069, 0.048 42.7 6.6 kDa lipoprotein (GI:1477781) 573 5.3 14.07 0.005, 0.255 55.0  
585 6.3 28.02 0.125, 0.010 12.2        
586 6.1 44.19 0.357, 0.001 674.8 *Flagellin (GI:120230)       
587 6.1 44.41 0.209, 0.000 765.7        
588 6.1 41.54 0.276, 0.001 527.4        
  1. * Flagellin appears to be produced at equivalent levels in both strains but fold change depicted is higher for respective spots due to slight mobility differences of this protein in B31 and N40D10/E9 gels.