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Table 1 Polypeptide spots that showed at least a 10-fold increase in relative abundance in B31 or N40D10/E9 on 2D protein gel

From: Comparative molecular analyses of Borrelia burgdorferi sensu stricto strains B31 and N40D10/E9 and determination of their pathogenicity

Spot #

pI

MW (kDa)

Relative abundance in B31, and N40 (%)

Fold change B31 vs N40

Identification MALDI-MS analyses (SwissProt or NCBI accession #)

Spot #

pI

MW (kDa)

Relative abundance in B31, and N40 (%)

Fold change N40 vs B31

Identification MALDI-MS analyses (SwissProt or NCBI accession #)

33

6.2

88.96

0.036, 0.003

11.2

 

136

5.6

64.58

0.002, 0.029

14.7

 

110

5.1

63.92

0.050, 0.003

15.1

 

208

5.8

53.07

0.015, 0.340

22.7

 

127

5.3

65.24

0.037, 0.003

11.5

 

231

6.9

52.81

0.019, 0.226

11.8

 

211

6.1

55,65

0.875, 0.048

18.0

 

272

6.2

46.29

0.000, 0.054

685.4

*Flagellin (GI:120230), Basic membrane protein A (GI:3913169)

225

6.1

57.07

0.193, 0.005

35.3

 

293

6.0

43.53

0.000, 0.170

698.2

*Flagellin (GI:120230)

325

5.6

38.32

0.114, 0.010

11.3

 

311

6.0

39.99

0.005, 0.165

30.6

 

403

5.4

31.03

0.071, 0.002

29.1

 

347

6.0

35.06

0.003, 0.185

59.8

 

404

5.4

31.00

0.404, 0.003

124.1

OspD (GI:495462)

348

5.6

34.95

0.007, 0.258

36.3

 

405

5.5

28.78

1.006, 0.031

32.7

 

349

6.0

34.36

0.003, 0.095

32.4

 

458

5.7

26.07

0.051, 0.003

15.2

 

352

6.5

34.25

0.003, 0.034

12.0

 

463

6.5

25.58

0.107, 0.007

15.4

 

354

5.9

34.62

0.004, 0.207

47.9

 

465

6.3

25.49

0.077, 0.006

13.2

 

381

5.6

29.61

0.003, 0.032

10.4

 

491

7.0

22.69

0.356, 0.012

29.8

 

406

6.2

28.54

0.006, 0.081

14.0

 

494

6.5

23.16

1.400, 0.062

22.8

 

418

6.3

27.97

0.089, 1.927

21.6

 

495

6.7

23.13

6.875, 0.025

278.1

Outer surface protein C (GI:3914248)

452

6.6

26.33

0.006, 0.246

42.5

30S ribosomal protein S4 (B7J2H5)

Phosphoglycolate phosphatase (GI:226320487), and hypothetical

(GI:226315606)

497

6.3

22.87

0.262, 0.022

12.1

 

479

6.4

24.51

0.060, 0.648

10.7

 

519

7.1

20.08

0.734, 0.027

26.8

 

501

6.5

22.47

0.030, 1.956

64.6

Same as 505

525

6.4

21.03

0.234, 0.008

30.9

Neutrophil activating protein (GI:15595035)

505

6.3

22.33

0.017, 0.570

34.0

OspC (GI: 226246807) Neutrophil activating protein (GI:15595035)

528

6.2

20.95

0.068, 0.004

15.9

 

517

6.2

21.41

0.002, 0.095

54.4

 

541

6.4

20.31

0.097, 0.005

20.8

 

543

5.6

19.67

0.006, 0.072

11.9

 

559

5.6

17.70

0.137, 0.008

17.2

 

551

6.2

19.51

0.075, 0.762

10.2

 

581

4.9

11.91

2.069, 0.048

42.7

6.6 kDa lipoprotein (GI:1477781)

573

5.3

14.07

0.005, 0.255

55.0

 

585

6.3

28.02

0.125, 0.010

12.2

       

586

6.1

44.19

0.357, 0.001

674.8

*Flagellin (GI:120230)

      

587

6.1

44.41

0.209, 0.000

765.7

       

588

6.1

41.54

0.276, 0.001

527.4

       
  1. * Flagellin appears to be produced at equivalent levels in both strains but fold change depicted is higher for respective spots due to slight mobility differences of this protein in B31 and N40D10/E9 gels.
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BMC Microbiology

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