Comparative molecular analysis of chemolithoautotrophic bacterial diversity and community structure from coastal saline soils, Gujarat, India

Table 2 Biodiversity and predicted richness of the cbbL and 16S rRNA gene sequences

Genes	No of clones	Coverage (%)	Evenness(J)	Shannon Weiner (H)	Simpson (1-D)	Sobs¹(OTU)	Chao	ACE	No of Singletons
cbbL form IC
AS	141	83	0.92	3.7	0.98	58	71.8	87.2	24
SS1	99	91	0.92	3.2	0.96	32	34.3	37.6	8
SS2	103	91	0.94	3.5	0.97	40	43.6	43.8	9
cbbL form IA
SS2	28	82	0.58	1.2	0.55	8	11.3	16.8	5
16S rRNA
AS	147	33	0.92	4.3	0.98	109	584.3	4626.3	98
SS1	97	56	0.92	3.7	0.97	55	206.5	553.5	41
SS2	85	36	0.93	3.9	0.97	63	311.5	1278.9	53

¹OTUs for cbbL-gene clone libraries were determined at a 0.05 distance cut-off and OTUs for 16S rRNA clone libraries were determined at a 0.02 cut-off using the MOTHUR program. The Coverage, Shannon-Weiner (H), Simpson (1-D), Evenness (J) indices and Chao & ACE richness estimators were calculated using the OTU data.

ISSN: 1471-2180