TLR4 expression in bladder cells after Lactobacillus stimulation. (A) TLR4 qPCR from cells co-stimulated for 3 h with E. coli and L. rhamnosus GR-1 (n = 3). (B) A native immunoblot of TLR4 protein after 24 h stimulation. (C) Confocal microscopy of TLR4 protein (green pixels) after stimulation of T24 cells. The cells were also stained with DAPI (DNA stain) and Alexa555 phalloidin (actin stain) and pseudo-colored blue and red, respectively. Immunoblot and confocal images are representative data from two or more separate experiments. Bars labeled with an asterisk are significantly different from control cells (p-values < 0.05).