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Table 2 Bacterial strains and plasmids used in this study

From: Role of the small RNA RyhB in the Fur regulon in mediating the capsular polysaccharide biosynthesis and iron acquisition systems in Klebsiella pneumoniae

Strains or plasmids Descriptions Reference or source
K. pneumoniae   
CG43S3 CG43 Smr [56]
ΔlacZ CG43S3ΔlacZ [17]
Δfur CG43S3Δfur [22]
ΔlacZ Δfur CG43S3ΔlacZ Δfur [22]
ΔryhB CG43S3ΔryhB This study
Δfur ΔryhB CG43S3Δfur ΔryhB This study
ΔlacZ Δfur ΔryhB CG43S3ΔlacZ Δfur ΔryhB This study
ΔgalU CG43S3ΔgalU [57]
E. coli   
DH5α supE44 ΔlacU169 (f80 lacZ Δμ15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [58]
BL21-RIL F-ompT hsdS B [r B -m B -]gal dcm [DE3] Laboratory stock
S17-1 λ pir H1717 hsdR recA pro RP4-2 [Tc::Mu; Km::Tn7] [λpir] araD139 ΔlacU169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB fhuF::λ placMu [59, 60]
Plasmids   
pKAS46 Positive selection suicide vector, rpsL Apr Kmr [59]
yT&A TA cloning vector Yeastern
pRK415 Broad-host-range IncP cloning vector, Tcr [61]
pT7-7 Cloning vector, Apr [62]
pETQ Kmr, protein expression vector [61]
placZ15 Cmr, promoter selection vector, lacZ+ [17]
pfur Tcr, 0.8-kb fragment containing a fur allele cloned into pRK415 [22]
pET30c-Fur Kmr, 450-bp fragment encoding full-length Fur cloned into pET30c [22]
pRyhB04 2.0 kb fragment containing an internal ~70-bp deletion in ryhB cloned into pKAS46 This study
pRyhB15 Cmr, 178-bp fragment containing the region upstream of ryhB cloned into placZ15 This study
pOrf12 Cmr, 500-bp fragment containing the region upstream of Klebsiella K2 cps orf1-orf2 cloned into placZ15 [17]
pOrf315 Cmr, 900-bp fragment containing the region upstream of Klebsiella K2 cps orf3-orf15 cloned into placZ15 [17]
pOrf1617 Cmr, 300-bp fragment containing the region upstream of Klebsiella K2 cps orf16-orf17 cloned into placZ15 [17]
pT7-7-pryhB 178-bp fragment containing the putative ryhB promoter, cloned into pT7-7 This study
pETQ-ryhB Kmr, 326-bp fragment containing the promoter and coding region of ryhB cloned into pETQ This study