Figure 5

BLI after Yp lux⁺intranasal inoculation in the left nostril of B6(Cg)- Tyrc-2J /J mice. (A) Mice were inoculated IN with ~10⁴ CFU.Images of the neck and head (dorsal and ventral) at 24 hpi under an individual radiance scale. The color bars serve as reference for radiance intensity (p/sec/cm²/sr; Min and Max values are shown) from each spot in the mouse from which signal was detected. (B) Images of the dorsal and ventral sides of the animals at different time points (shown in hpi). (C) Signal from the lungs after dissection in an animal infected ID in comparison to an animal infected IN (Min = 5.02e7 and Max = 8.62e8). (D) Isolated lungs showing a necrotic spot (photograph) and how highest levels of radiance (photograph + luminescence) originated from this spot (Min = 4.42e6 and Max = 7.02e8). Color bars serve as reference for radiance values. Shown is a representative experiment