BLI of C57BL/6J mice infected subcutaneously with Yp lux+at a cervical site. (A) Animals were inoculated with ~200 CFU and imaged at the indicated hours post inoculation (hpi). Luminescence signal is reported as radiance (p/sec/cm2/sr) in a scale paired with a color bar shown next to the images. For 6 hpi, the image in the window is shown using an individual color scale with radiance of Min = 8.53e3 and Max = 3.97e4. (B) Images of the abdomen at 72 and 96 hpi (same mice shown in panel A) under an individual radiance scale (Max and Min values are shown). (C) Site of inoculation [skin (inner side)], superficial cervical lymph nodes, spleen and liver (from one of the mice shown in A) imaged individually after dissection. Individual scales of radiance were used due to variability in signal (site of infection and liver, Min = 1.57e5 Max = 3.74e6; lymph nodes, Min = 2.10e6 Max = 2.28e8; spleen, Min = 1.73e5 Max = 1.38e7). Shown is a representative experiment.