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Table 3 Effects of bacterial probiotic supplementation on rumen fermentation characteristics during acidosis induced by feed challenges

From: Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep

 

Treatments1

 

 P  value (Prob vs.  C)2

 

C (n = 4)

 P (n = 4)

 Lp + P (n = 4)

 Lr + P (n = 4)

SEM

 P 

 Lp + P 

 Lr + P 

Wheat-induced lactic acidosis

Ruminal pH

        

Mean

5.25

4.55

4.76

4.33

0.15

0.001

0.02

0.0001

Minimum

4.87

4.28

4.45

4.17

0.19

0.03

0.12

0.01

Total VFAs, mM

93.6

33.9

76.7

33.5

14.4

0.01

0.32

0.001

Acetate3, mol %

72.6

87.0

78.1

92.5

4.10

0.01

0.34

0.001

Propionate, mol %

12.2

6.63

10.6

3.82

2.49

0.10

0.63

0.02

Butyrate, mol %

12.8

5.79

10.2

3.52

1.94

0.01

0.33

0.001

Minor VFAs4, mol %

2.33

0.56

1.11

0.14

0.40

0.001

0.02

0.0001

Lactate, mM

33.8

71.1

64.9

79.6

9.28

0.005

0.02

0.001

NH3-N, mM

6.53

3.58

4.25

2.44

1.16

0.03

0.09

0.003

Ethanol, mM

6.57

12.4

17.2

14.4

1.85

0.02

0.0001

0.003

Corn-induced butyric subacute acidosis

Ruminal pH

        

Mean

5.49

5.61

5.74

5.65

0.08

0.30

0.03

0.18

Minimum

5.17

5.28

5.63

5.46

0.12

0.50

0.01

0.09

Total VFAs, mM

107

85.7

81.6

94.4

7.79

0.03

0.01

0.19

Acetate, mol %

63.2

67.4

68.7

66.9

1.75

0.08

0.03

0.13

Propionate, mol %

17.0

14.2

14.5

15.5

1.09

0.07

0.19

0.31

Butyrate, mol %

16.9

14.7

12.1

13.5

1.41

0.26

0.02

0.09

Minor VFAs, mol %

2.88

3.68

4.29

4.09

0.44

0.20

0.02

0.05

Lactate, mM

3.40

3.78

3.22

3.49

0.86

0.71

0.87

0.92

NH3-N, mM

0.74

0.73

0.71

1.15

0.98

0.99

0.98

0.76

Ethanol, mM

3.15

3.60

2.72

2.74

0.36

0.38

0.40

0.42

Beet pulp-induced propionic subacute acidosis

Ruminal pH

        

Mean

5.67

5.94

5.87

5.93

0.08

0.02

0.08

0.02

Minimum

5.55

5.84

5.72

5.83

0.11

0.05

0.27

0.06

Total VFAs, mM

114

112

104

100

6.66

0.89

0.33

0.16

Acetate, mol %

67.4

68.6

68.4

67.8

1.15

0.46

0.55

0.79

Propionate, mol %

22.5

21.5

21.9

22.3

0.83

0.38

0.61

0.88

Butyrate, mol %

8.52

8.40

8.18

8.34

0.49

0.86

0.85

0.77

Minor VFAs, mol %

1.50

1.48

1.52

1.46

0.26

0.94

0.96

0.91

Lactate, mM

2.71

2.01

1.52

2.01

1.46

0.73

0.56

0.73

NH3-N, mM

0.55

0.51

0.57

0.57

0.74

0.97

0.99

0.98

Ethanol, mM

3.34

3.22

2.64

2.84

0.48

0.86

0.31

0.47

  1. 1 Treatment with C = control without probiotic; P = Propionibacterium P63; Lp + P = L. plantarum + P63; Lr + P = L. rhamnosus + P63. 2 Effect of each probiotic treatment vs. control wether (C). 3 Individual VFAs are expressed in % of total VFAs. 4 Minor VFAs: sum of iso-butyrate, iso-valerate, valerate and caproate. The fermentation characteristics were determined on d3 at 6 h after feed challenges induced acidosis.