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Table 3 Effects of bacterial probiotic supplementation on rumen fermentation characteristics during acidosis induced by feed challenges

From: Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep

  Treatments1    P  value (Prob vs.  C)2
  C (n = 4)  P (n = 4)  Lp + P (n = 4)  Lr + P (n = 4) SEM  P   Lp + P   Lr + P 
Wheat-induced lactic acidosis
Ruminal pH         
Mean 5.25 4.55 4.76 4.33 0.15 0.001 0.02 0.0001
Minimum 4.87 4.28 4.45 4.17 0.19 0.03 0.12 0.01
Total VFAs, mM 93.6 33.9 76.7 33.5 14.4 0.01 0.32 0.001
Acetate3, mol % 72.6 87.0 78.1 92.5 4.10 0.01 0.34 0.001
Propionate, mol % 12.2 6.63 10.6 3.82 2.49 0.10 0.63 0.02
Butyrate, mol % 12.8 5.79 10.2 3.52 1.94 0.01 0.33 0.001
Minor VFAs4, mol % 2.33 0.56 1.11 0.14 0.40 0.001 0.02 0.0001
Lactate, mM 33.8 71.1 64.9 79.6 9.28 0.005 0.02 0.001
NH3-N, mM 6.53 3.58 4.25 2.44 1.16 0.03 0.09 0.003
Ethanol, mM 6.57 12.4 17.2 14.4 1.85 0.02 0.0001 0.003
Corn-induced butyric subacute acidosis
Ruminal pH         
Mean 5.49 5.61 5.74 5.65 0.08 0.30 0.03 0.18
Minimum 5.17 5.28 5.63 5.46 0.12 0.50 0.01 0.09
Total VFAs, mM 107 85.7 81.6 94.4 7.79 0.03 0.01 0.19
Acetate, mol % 63.2 67.4 68.7 66.9 1.75 0.08 0.03 0.13
Propionate, mol % 17.0 14.2 14.5 15.5 1.09 0.07 0.19 0.31
Butyrate, mol % 16.9 14.7 12.1 13.5 1.41 0.26 0.02 0.09
Minor VFAs, mol % 2.88 3.68 4.29 4.09 0.44 0.20 0.02 0.05
Lactate, mM 3.40 3.78 3.22 3.49 0.86 0.71 0.87 0.92
NH3-N, mM 0.74 0.73 0.71 1.15 0.98 0.99 0.98 0.76
Ethanol, mM 3.15 3.60 2.72 2.74 0.36 0.38 0.40 0.42
Beet pulp-induced propionic subacute acidosis
Ruminal pH         
Mean 5.67 5.94 5.87 5.93 0.08 0.02 0.08 0.02
Minimum 5.55 5.84 5.72 5.83 0.11 0.05 0.27 0.06
Total VFAs, mM 114 112 104 100 6.66 0.89 0.33 0.16
Acetate, mol % 67.4 68.6 68.4 67.8 1.15 0.46 0.55 0.79
Propionate, mol % 22.5 21.5 21.9 22.3 0.83 0.38 0.61 0.88
Butyrate, mol % 8.52 8.40 8.18 8.34 0.49 0.86 0.85 0.77
Minor VFAs, mol % 1.50 1.48 1.52 1.46 0.26 0.94 0.96 0.91
Lactate, mM 2.71 2.01 1.52 2.01 1.46 0.73 0.56 0.73
NH3-N, mM 0.55 0.51 0.57 0.57 0.74 0.97 0.99 0.98
Ethanol, mM 3.34 3.22 2.64 2.84 0.48 0.86 0.31 0.47
  1. 1 Treatment with C = control without probiotic; P = Propionibacterium P63; Lp + P = L. plantarum + P63; Lr + P = L. rhamnosus + P63. 2 Effect of each probiotic treatment vs. control wether (C). 3 Individual VFAs are expressed in % of total VFAs. 4 Minor VFAs: sum of iso-butyrate, iso-valerate, valerate and caproate. The fermentation characteristics were determined on d3 at 6 h after feed challenges induced acidosis.