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Table 2 Redox potentials of the assay buffers

From: Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of Escherichia coli K-12

Hydrogen in headspace 50 mM MOPS, pH 7 50 mM MOPS, pH 7, BV/TTCa 50 mM MOPS, pH 7, PMS/NBTb 50 mM MOPS, pH 7, NBT
0%c + 170 mV + 78 mV + 74 mV + 73 mV
5% - 120 mV - 264 mV - 38 mV - 65 mV
100% - 349 mV - 322 mV - 92 mV - 102 mV
  1. a The concentrations of BV and TTC were 0.5 mM and 1.0 mM, respectively.
  2. b The concentrations of PMS and NBT were 0.3 mM and 0.2 mM, respectively.
  3. c Measured at 25 °C and 1 atm. pressure. 0% hydrogen indicates measurements were made in air. Note that all measurements were made twice.