Figure 1

Identification of hydrogenases 1 and 2 in defined hydrogen metabolism mutants. Extracts from strains HDK203 (ΔhybBC hycA-H), which is Hyd-1⁺, HDK101 (Δhya hycA), which is Hyd-2⁺ and Hyd-3⁺ and HDK103 (Δhya hycA-H), which is Hyd-2⁺ were derived from cells after anaerobic growth in TGYEP, pH 6.5 and 25 μg of protein were applied to non-denaturating PAGE (7.5% w/v polyacrylamide). After electrophoresis the gel was stained in an anaerobic glove box in the presence of ≤5% H₂ with BV and TTC as described in the Methods section. On the right hand side of the figure the migration patterns of the formate dehydrogenases N and O (Fdh-N/O) and the hydrogenases (Hyd) 1 and 2 are given. The top of the gel is marked by an arrow.