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Table 3 Summarised Purification steps of ACP

From: Biochemical characterization of an anti-Candida factor produced by Enterococcus faecalis

Purification stage Volume (mL) Activity (AU mL-1) Protein (mg mL-1) Specific activity (AUmg-1protein) Purification factor Recovery (%)
Culture Supernatant 400 1600 0.4025 39751 1 100
Ammonium sulfate and dialysis 10 3200 0.0444 72072 1.8 11
Ion Exchange Chromatography 6 1600 0.0023 695652 17.5 0.57
Gel Filtration 2 1600 0.0018 888888 22.4 0.45