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Table 2 Effect of enzymes, heat, pH, organic solvents and surfactants on the biological activity of ACP (+ve sign, biological activity retained, -ve sign, loss of biological activity)

From: Biochemical characterization of an anti-Candida factor produced by Enterococcus faecalis

Treatment (w/v) Activity Treatment (v/v) Activity
Trypsin (1.0 mg ml-1) + Methanol (25%) +
Pronase E (1.0 mg ml-1) - Ethanol (25%) +
Proteinase K (1.0 mg ml-1) -- Iso-propanol (10%) +
Pepsin (1.0 mg ml-1) + Hexane (25%) +
α-Amylase (1.0 mg ml-1) + Formaldehyde (10%) +
Lipase (1.0 mg ml-1) + Chloroform (10%) +
Lysozyme (2.0 mg ml-1) + Acetone (10%) +
37°C, 60°C for 90 min + Acetonitrile (70%) +
90°C for 20 min + Triton X-100 (1%v/v) +
100°C for 30 min - Tween-20 (1%v/v) +
100°C for 90 min - SDS (1%w/v) ++
121°C for 15 min - Urea (1%w/v) +
Control at 4°C + EDTA (1%w/v) +
(pH) 6.0, 7.0 and 8.0 + PMSF (1%v/v) +
(pH) 2.0, 4.0 and 10.0 - β-Mercaptoethanol (1 mmol) +
   DTT (0.1 mol) +