Table 2 Effect of enzymes, heat, pH, organic solvents and surfactants on the biological activity of ACP (+ve sign, biological activity retained, -ve sign, loss of biological activity)
From: Biochemical characterization of an anti-Candida factor produced by Enterococcus faecalis
Treatment (w/v) | Activity | Treatment (v/v) | Activity |
|---|---|---|---|
Trypsin (1.0 mg ml-1) | + | Methanol (25%) | + |
Pronase E (1.0 mg ml-1) | - | Ethanol (25%) | + |
Proteinase K (1.0 mg ml-1) | -- | Iso-propanol (10%) | + |
Pepsin (1.0 mg ml-1) | + | Hexane (25%) | + |
α-Amylase (1.0 mg ml-1) | + | Formaldehyde (10%) | + |
Lipase (1.0 mg ml-1) | + | Chloroform (10%) | + |
Lysozyme (2.0 mg ml-1) | + | Acetone (10%) | + |
37°C, 60°C for 90 min | + | Acetonitrile (70%) | + |
90°C for 20 min | + | Triton X-100 (1%v/v) | + |
100°C for 30 min | - | Tween-20 (1%v/v) | + |
100°C for 90 min | - | SDS (1%w/v) | ++ |
121°C for 15 min | - | Urea (1%w/v) | + |
Control at 4°C | + | EDTA (1%w/v) | + |
(pH) 6.0, 7.0 and 8.0 | + | PMSF (1%v/v) | + |
(pH) 2.0, 4.0 and 10.0 | - | β-Mercaptoethanol (1 mmol) | + |
| Â | Â | DTT (0.1 mol) | + |