Figure 6
Pycnidia development (accompanied by asexual sporulation) in the S. nodorum wild-type strain SN15 is observed in a distinct circadian ring pattern (A and B) within 5 days post inoculation (dpi) of solid minimal medium*. Pycnidia do not develop in the mutant strains during this timeframe. The formation of pycnidia in the S. nodorum mutant strains gna 1-35, gba 1-6 and gga1-25 is evident amongst the outer mycelia (C - E) from between 3 and 6 weeks incubation of (the initially) non-sporulating (5 dpi) culture at 4°C. S. nodorum strains are pictured growing on nitrocellulose membranes (30 mm diameter)-overlaying minimal medium agar.