Table 3 Oligonucleotides used in this study
Name | Sequence | Purpose |
|---|---|---|
1 | GAGATATGGTTATGAGATTAGG | Presence/absence of insert |
2 | CCCACCTTTATAGCATCATATAG | Absence of insert |
3 | CTAACCTATCAACTCAACCCC | Presence of insert |
4 | AGGATAAGACCGCAGCAGAA | Presence 5’half of insert |
5 | AAAAACGACGGTTTTCCTGTG | Presence 5’half of insert |
6 | GGGCAAATAGAAAGTCAAAACG | Presence 3’half of insert |
7 | AAGTGGTGTTTTCTTTGGAGGA | Presence 3’half of insert |
8 | CCACAGGGATACCTTTCTCGTGC | Presence of tet(44) gene |
9 | TTCCATATCCTCGGGTTTTTGCAT | Presence of tet(44) gene |
10 | CAGGTGTTGAAATAGATATTGAG | Detect 3' end half insert |
11 | CAGAAGTCGATCCTTTCTGGG | Detect 3' end half insert |
12 | GGTGGCTGAACTCGTTAATC | Detect 3' end half insert |
13 | CTCCACATGGCTCGAGTTG | Detect 3' end half insert |
14 | GAGGAATTTAACAGAACAGTATTT | Excision studies |
15 | TCTATCCTGCCTTCTCAACC | Excision studies |
16 | CGAATCGCTGAAATGACTGA | Excision studies |
17 | GCGAATGATTTCATGGAAGG | Excision studies |
18 | CGACTGCATTACCAGTTCCA | Excision studies |
Lok1 [13] | AAAATATACTGCACATCTGTATAC | Transconjugant screening |
Lok3 [13] | TTTACCAGAAAAAGTAGCTTTAA | Transconjugant screening |
19 | CAGCTGCAGTTTTTCCATGA | Transconjugant screening |
20 | GCAGCTAACGGTGATGACAA | Transconjugant screening |
Tn916 Fw [30] | GACGGAAGATACTTATACA | Transconjugant screening |
Tn916 Rev [30] | GCCTTTGGATTCATTCCTGC | Transconjugant screening |