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Table 2 Detection of specific regions of Tn6164 in PCR ribotype 078 strains

From: Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164

Strain PCR 1-2* PCR1-3§ PCR 4-5# PCR 6-7 PCR 8-9 PCR 12-2‡
56/69 - + + - - +
26222 - + + - - +
26114 - + + - - +
26247 - + + - - +
26235 - + + - - +
ES1203 - + + - - n.t.
6065935 - + + - n.t. n.t.
7047337 - + + - n.t. n.t.
8088158 - + + - n.t. n.t.
50/19 - + + + + -
GR0106 - + + + + n.t.
DE1210 - + + + + n.t.
BG1209 - + + + + n.t.
NO1311 - + + + + n.t.
NO1307 - + + + + n.t.
IE1102 - + + + + n.t.
GR0301 - + + + + n.t.
10053737 - + + + n.t. n.t.
  1. *PCR only positive when no insert is present, §PCR only positive when insert is present #PCR detects Module B, PCR detects module E, PCR detects module D. ‡ PCR only positive in strains containing half of the element. Location of the oligonucleotides used is indicated in Figure 1. +, PCR positive; -, PCR negative; n.t., not tested.