Table 2 Kinetic parameters of the recombinant Aad1p from Phanerochaete chrysosporium
K M μM | K cat min-1 | k cat /K M μM-1·min-1 | K i μM | |
|---|---|---|---|---|
Substrates | ||||
Reduction | ||||
3,4-Dimethoxybenzaldehyde | 12 ± 2 | 530 ± 25 | 44 ± 9 | 3400 ± 1100 |
3,5-Dimethoxybenzaldehyde | 22 ± 4 | 590 ± 30 | 27 ± 6 | 2100 ± 600 |
4-Methoxybenzaldehyde | 90 ± 10 | 490 ± 10 | 5.4 ± 0.7 | ni |
5-(Hydroxymethyl)-2-furaldehyde | 270 ± 40 | 176 ± 6 | 0.65 ± 0.12 | 136000 ± 28000 |
Phenylacetaldehyde | 530 ± 90 | 670 ± 25 | 1.3 ± 0.3 | ni |
3-Hydroxy-4-methoxybenzaldehyde | 1400 ± 900 | 230 ± 110 | 0.16 ± 0.18 | 2300 ± 1800 |
4-Hydroxy-3-methoxybenzaldehyde | 1400 ± 600 | 200 ± 50 | 0.14 ± 0.10 | 5100 ± 2300 |
Benzaldehyde | 1700 ± 600 | 430 ± 50 | 0.3 ± 0.1 | 81000 ± 44000 |
trans-Cinnamaldehyde | 3400 ± 1300 | 670 ± 200 | 0.2 ± 0.1 | 3500 ± 1600 |
Oxidation | ||||
3,4-Dimethoxybenzyl alcohol | 370 ± 50 | 153 ± 6 | 0.41 ± 0.07 | 165000 ± 31000 |
4-Hydroxy-3-methoxybenzyl alcohol | 25000 ± 7000 | 260 ± 60 | 0.010 ± 0.005 | ni |
Coenzymes | ||||
Oxidation | ||||
NADPH | 39 ± 5 | 680 ± 30 | 17 ± 3 | ni |
NADH | 220 ± 130 | 120 ± 40 | 0.6 ± 0.5 | ni |
Reduction | ||||
NADP+ | 38 ± 7 | 154 ± 7 | 4.1 ± 0.9 | ni |
NAD+ | nd | nd | nd | nd |