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Table 1 Substrate specificity of the recombinant Aad1p from Phanerochaete chrysosporium

From: Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767

Reduction

Activity (%)

Oxidation

Activity (%)

Linear aliphatic aldehydes

 

Aryl-alcohols

 

 Propanal (C3)

nd

 4-Methoxybenzyl alcohol

15

 Butanal (C4)

nd

 3,4-Dimethoxybenzyl alcohol

100

 Pentanal (C5)

8

 3,5-Dimethoxybenzyl alcohol

3

 Hexanal (C6)

47

 3,4,5-Trimethoxybenzyl alcohol

5

 Heptaldehyde (C7)

26

 4-Hydroxybenzyl alcohol

3

 Octanal (C8)

37

 3-Hydroxy-4-methoxybenzyl alcohol

8

 Nonanal(C9)

3

 4-Hydroxy-3-methoxybenzyl alcohol

46

 Decanal (C10)

2

  

 Undecanal (C11)

1

  

Aryl-aldehydes

 

No detectable activity

 

 Benzladehyde

42

Linear aliphatic alcohols

 

 2-Methoxybenzaldehyde

58

  Ethanol (C2)

 

 3-Methoxybenzaldehyde

117

  Propanol (C3)

 

 4-Methoxybenzaldehyde

110

  Butanol (C4)

 

 3,4-Dimethoxybenzaldehyde

100

  Pentanol (C5)

 

 3,5-Dimethoxybenzaldehyde

110

  Hexanol (C6)

 

 4-Hydroxybenzaldehyde

34

  Octanol (C8)

 

 3-Hydroxy-4-methoxybenzaldehyde

17

  Nonanol (C9)

 

 4-Hydroxy-3-methoxybenzaldehyde

19

  Decanol (C10)

 

 3-Chlorobenzaldehyde

73

  Undecanol (C11)

 

 4-Chlorobenzaldehyde

61

Ramified aliphatic alcohols

 

 2-Nitrobenzaldehyde

57

  2-Methylpropanol (C4)

 

 3-Nitrobenzaldehyde

25

  2-Methylbutanol (C5)

 

 4-Nitrobenzaldehyde

31

Aryl-alcohols

 

 2-Fluorobenzaldehyde

27

  Benzyl alcohol

 

 3-Fluorobenzaldehyde

69

  2-Methylbenzyl alcohol

 

 4-Fluorobenzaldehyde

29

  3-Methylbenzyl alcohol

 

 Phenylacetaldehyde

109

  4-Methylbenzyl alcohol

 

trans-Cinnamaldehyde

39

  2-Methoxybenzyl alcohol

 

Others

 

  3-Methoxybenzyl alcohol

 

 5-(Hydroxymethyl)-2-furaldehyde

36

  2-Chlorobenzyl alcohol

 
  

  4-Chlorobenzyl alcohol

 

No detectable activity

 

  2-Nitrobenzyl alcohol

 

Aryl-acid

 

  3-Nitrobenzyl alcohol

 

  Phenylacetic acid

 

  4-Nitrobenzyl alcohol

 

Ethyl-esters

 

  2-Fluorobenzyl alcohol

 

  Ethyl hexanoate

 

  3-Fluorobenzyl alcohol

 

  Ethyl octanoate

 

  4-Fluorobenzyl alcohol

 

  Ethyl decanoate

 

  2-Phenylethanol

 

Ramified and aryl acetate esters

 

  trans-Cinnamyl alcohol

 

  2-Methylpropyl acetate

 

Aryl-acid

 

  2-Methylbutyl acetate

 

  Phenylacetic acid

 

  3-Methylbutyl acetate

 

Ethyl-esters

 

  2-Phenylethyl acetate

 

  Ethyl hexanoate

 

Others

 

  Ethyl octanoate

 

  L-Glutathione oxidized

 

  Ethyl decanoate

 
  

Ramified and aryl acetate esters

 
  

  2-Methylpropyl acetate

 
  

  2-Methylbutyl acetate

 
  

  3-Methylbutyl acetate

 
  

  2-Phenylethyl acetate

 
  1. Results are the mean of three separate experiments with relative SEM being lower than 6%.