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Figure 2 | BMC Microbiology

Figure 2

From: Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767

Figure 2

Purification of the recombinant Pc Aad1p after expression in E. coli. The Pc Aad1p fused to GST and His6 tags was expressed in E. coli BL21 Star™(DE3) strain with the pGS-21a expression vector under the control of the strong T7 promoter. Proteins were separated by SDS-PAGE and visualized by Coomassie Blue staining. Lane 1: Cell lysate of E. coli IPTG-induced cells; Lane 2: Protein molecular size markers; Lane 3: Recombinant Pc Aad1p after purification by Glutathione-affinity chromatography.

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