Table 2 Estimation (%) and enrichment of Sulfur and Nitrogen biochemical functional genes in wastewater genomes

Single-copy genes ^‡

5

100

100

ns

1.0

1.0

Sulfur metabolism

Sulfate adenylyltransferase (ATP)

cysN

1

54

33

0.000

1.6

0.6

Adenylyl-sulfate kinase

aspK

1

52

15

0.000

3.2

0.3

Phosphoadenylyl-sulfate reductase

cysH

1

26

22

ns

1.1

0.9

Adenylyl-sulfate reductase

aprA

1

15

10

ns

1.4

0.7

3'(2'),5'-bisphosphate nucleotidase

cysQ

1

67

40

0.000

1.6

0.6

Hydrogensulfite reductase

dsrA

1

13

15

ns

0.8

1.3

Sulfite reductase (NADPH)

cysJ

1

28

4

0.000

7.6

0.1

Sulfite reductase (DSR)

dsrB

1

13

14

ns

1.0

1.0

Sulfite reductase (ferredoxin)

sir

1

22

6

0.000

3.7

0.3

Cysteine synthase

cysK

1

>100

>100

ns

1.0

1.0

Thiosulfate oxidise

soxB

1

66

7

0.000

9.1

0.1

Nitrogen metabolism

Ammonia monooxygenase

amoA

1

8

29

0.000

0.3

3.6

Nitrate reductase

napA

1

2

13

0.000

0.1

8.0

Nitrate reductase

narG

1

17

28

0.000

0.6

1.7

Nitrate reductase

nasA

1

68

34

0.000

2.0

0.5

Nitric oxide reductase

norB

1

2

23

0.001

0.1

9.4

Nitric oxide reductase

qnor

1

22

23

ns

1.0

1.0

Nitrite reductase

nirK

1

17

3

0.000

5.2

0.2

Nitrite reductase

nirS

1

2

30

0.000

0.1

16.4

Nitrous oxide reductase

nosZ

1

10

35

0.030

0.3

3.6

Nitrite reductase

nirB

1

64

44

0.000

1.4

0.7

Nitrite reductase

nirA

1

7

1

0.018

5.6

0.2

Nitrite reductase

nrfA

1

1

45

0.000

0.0

58.4

Nitrogenase (molybdenum-iron)

nifD

1

1

23

0.000

0.0

24.6

Nitrogenase (iron)

nifH

1

15

23

0.006

0.6

1.6