Metagenome analyses of corroded concrete wastewater pipe biofilms reveal a complex microbial system

BMC Microbiology

Table 1 Characterization of 454 pyrosequenced libraries from the microbial community of biofilms

	Top pipe (TP)	Bottom pipe (BP)
reads	1 004 530	976 729
avg reads (bp)	370	427
dataset size (10⁸ bp)	3.2	3.7
reads for analysis^§	862 893	856 080
CAMERA v2
COG hits^†	370 393	389 807
Pfam hits^†	338 966	352 466
TIGRfam hits^†	579 127	607 388
MG-RAST v3
reads matching to a taxa^†	629 161	641 853
reads matching to a subsystems^†	425 346	427 295
no. of subsystems (function level)	5 633	6 117
Annotated proteins (%) [SEED]
Bacteria	95.5	94.1
Archaea	0.5	1.3
Virus	0.1	0.1
Eukaryota	0.6	0.3
Unclassified	3.3	4.2
Comparative metagenome ^‡
average genome size [Mb]	3.3	3.3
ESC of COG hits	369 671	390 570

^§Prior to sequence analysis we implemented a dereplication pipeline to identify and remove clusters of artificially replicated sequences [17].
^†E-value cut-off >1e^-05.
^‡Average genome size and effective sequence count (ESC) as calculated by Beszteri et al.[20].

ISSN: 1471-2180