Table 1 Plasmids and oligonucleotide primers
Plasmid | Characteristics | Source or Reference |
|---|---|---|
pCR2.1-TOPO | Cloning vector, kanamycin resistance and ampicillin resistance genes | Invitrogen |
pCP0 | Vector for gene expression in mycobacteria, kanamycin resistance gene | [4] |
pCP0-gplH | pCP0 expressing M. smegmatis gplH | This study |
pCP0-mbtHMs | pCP0 expressing M. smegmatis mbtH (MSMEG_4508) | [35] |
p2NIL | Kanamycin resistance gene and OriE | [57] |
pGOAL19 | Hygromycin resistance gene, sacB-lacZ PacI cassette, and OriE | [57] |
p2NIL-GOALc-ΔgplHc | Delivery vector carrying a gplH deletion cassette (ΔgplHc) | This study |
Oligonucleotide | Sequence (5’ to 3’) | Characteristics |
pepOF | GGTACCTGTTCAACGCGGCCAGAGCGTCATTGGTCTCGGCCA | KpnI |
pepOR | TTAATTAATGTTGCAACAGCTCCCTGATCCGGATGTCGACGTGCTTG | PacI |
pepIR | TCAGCCGTCAAGAGCAAAGCTGCCGTTGTCGTCATCGAACGGGTTGAT | SOE PCR |
pepIF | CGACAACGGCAGCTTTGCTCTTGACGGCTGAGTCAAATAGTCTGTTG | SOE PCR |
pepF | CTGCAGTGAACAGCCGGGAGAAACGT | PstI |
pepR | AAGCTTCCCAACAGACTATTTGACTCAGCCG | HindIII |