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Figure 1 | BMC Microbiology

Figure 1

From: Transcriptional analysis of the Escherichia coli ColV-Ia plasmid pS88 during growth in human serum and urine

Figure 1

Linearity and reproducibility of transcriptional analysis. (A) Quantitative RT-PCR of 5 ORFs using different RNA concentrations. (B) Analysis of fold changes in RNA transcript abundance by the 2-ΔΔCT method across 3 biological replicates named experiments 1, 2 and 3 after growth in LB broth (experiment 1 vs 2: dots, experiment 1 vs 3: squares, experiment 2 vs 3: triangles). The fold changes fall within the range 0.25-4.00 in 98% of cases.

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