Molecular analysis of volatile metabolites released specifically by staphylococcus aureus and pseudomonas aeruginosa

Filipiak, Wojciech; Sponring, Andreas; Baur, Maria Magdalena; Filipiak, Anna; Ager, Clemens; Wiesenhofer, Helmut; Nagl, Markus; Troppmair, Jakob; Amann, Anton

doi:10.1186/1471-2180-12-113

Table 2 Median concentrations of VOCs released or consumed by Staphylococcus aureus

From: Molecular analysis of volatile metabolites released specifically by staphylococcus aureus and pseudomonas aeruginosa

			median concentrations [ppbv]
Compound	CAS	m/z for SIM	medium	1.5 h	3.0 h	4.5 h	6.0 h
propanal	123-38-6	57	3.955	*10.62*	*14.22*	*8.932*	*7.04*
3-methyl-2-butenal	107-86-8	55, 84	1.526	1.832	*3.415*	*5.708*	*5.348*
2-ethylacrolein	922-63-4	84	1.656	2.01	*6.453*	*5.537*	*5.775*
(Z)-2-methyl-2-butenal	1115-11-3	84	73.48	81.91	*177.4*	*268.5*	*247.9*
(E)-2-methyl-2-butenal	497-03-0	84	< LOD	< LOD	*0.259*	*0.394*	*0.381*
benzaldehyde ^§	100-52-7	107	20.64	19.08	17.65	12.66	3.815
methacrolein	78-85-3	70	5.922	5.644	*9.328*	7.617	6.36
acetaldehyde	75-07-0	43	528.5	606.4	*374.2*	*1022.7*	*1417.4*
3-methylbutanal **	590-86-3	-	317.1	*403.3*	*2764.3*	*4779.3*	*4818.5*
2-methylpropanal **	78-84-2	₋	598.6	658.5	*2044.5*	*1698.6*	*1299.5*
1-butanol	71-36-3	56	< LOD	< LOD	< LOD	*21.24*	*59.4*
2-methyl-1-propanol	78-83-1	56, 74	0	0	0	*21.32*	*52.62*
3-methyl-1-butanol	123-51-3	55, 70	0	0	0	*27.65*	*210.0*
ethanol **	64-17-5	-	0	*89.57*	*237.0*	*6173.0*	*11695.1*
acetoin (hydroxybutanone)	513-86-0	88	< LOD	_3.59	_8.004	*140.6*	*279.3*
acetol (hydroxyacetone)	116-09-6	74	< LOD	< LOD	< LOD	*113.5*	*331.0*
2,3-butanedione	431-03-8	86	22.65	23.92	27.45	*49.84*	*67.99*
acetic acid	64-19-7	45, 60	0	0	0	*880.5*	*2566.6*
isovaleric acid	503-74-2	60	0	0	0	*31.13*	*97.35*
ethyl acetate	141-78-6	61	0	0	0	*1.973*	*5.624*
n-butyl acetate	123-86-4	56, 73	0	0	0	0	*0.239*
ethyl isovalerate	108-64-5	70	0	0	0	< LOD	*0.852*
isopentyl acetate	123-92-2	55, 70	0	0	0	< LOD	*1.938*
ethyl formate	109-94-4	31	0	0	0	< LOD	*3.188*
methyl methacrylate **	80-62-6	-	15.99	14.79	20.27	*28.65*	*31.93*
methanethiol	74-93-1	47	134.2	*210.4*	*360.6*	*559.4*	*701.5*
dimethyldisulfide (DMDS)	624-92-0	94	1.558	2.221	*3.657*	*8.134*	*10.24*
1,3-butadiene	106-99-0	54	< LOD	< LOD	*4.941*	*4.342*	*4.313*
2-methylpropene	115-11-7	56	< LOD	< LOD	*4.546*	*14.31*	*21.89*
n-butane	106-97-8	58	0.664	0.703	*1.274*	*2.504*	*4.329*
(Z)-2-butene	590-18-1	56	0	0	< LOD	*3.687*	*4.789*
(E)-2-butene	624-64-6	56	1.344	< LOD	*4.793*	*11.32*	*13.73*
propane	74-98-6	43, 41	0.91	0.815	1.951	*3.441*	*4.902*

Bold numbers indicate significant difference (Kruskal-Wallis test) in VOC concentrations between bacteria cultures and medium headspace (p < 0.05). Ethanol, 2-methylpropanal, 3- methylbutanal and methyl methacrylate were analyzed in TIC mode as indicated by **, while the remaining compounds were analyzed in SIM mode. Number of independent experiments n = 5 for each time point of bacteria growth, n = 14 for all medium controls. Concentrations are given in ppb_v, ^§ uptake (decreased concentration).

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ISSN: 1471-2180

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