A previously uncharacterized gene stm0551 plays a repressive role in the regulation of type 1 fimbriae in Salmonella enterica serotype Typhimurium

Table 1 Bacterial strains and plasmids used in this study

Name	Description ^a	Reference or source
Salmonella enterica serotype Typhimurium
LB5010	Wild type S. enterica serotype Typhimurium LT2 strain, fimbriate with the complete fim gene cluster	[21]
Δstm0551	stm0551 deletion mutant; Kan^r	Present study
Escherichia coli strain
One Shot® TOP10 chemically competent E. coli	F^-mcr A Δ(mrr-hsd RMS-mcr BC) Φ80lacZ ΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (Str^R) end A1 nup G	Invitrogen
BL21Star™ (DE3) One Shot® chemically competent E. coli	F^-ompT hsdS_B(r_B^- m_B^-) gal dcm (DE3)	Invitrogen
Plasmids
pSTM0551	A 0.5-kb DNA fragment possessing the stm0551 coding sequence cloned into the pACYC184 vector; Cm^r	Present study
pSTM0551E49A	A 0.5-kb DNA fragment possessing the stm0551 coding sequence with glutamic acid (E) at position 49 replaced with an alanine (A) cloned into the pACYC184 vector; Cm^r	Present study
pACYC184	Tet^r, Cm^r, cloning vector; w/p15A ori	ATCC, Manassas, VA
pET101/D-TOPO	Amp^r, 6xHis tag expression vector	Invitrogen
pKD46	Amp^r, express λ Red recombinase system, temp- sensitive replicon	[22]
pKD13	Plasmid carrying Kan^r cassette	[22]

^aAmp^r ampicillin resistant; Cm^r chloramphenicol resistant; Kan^r kanamycin resistant; Tet^r tetracycline resistant

ISSN: 1471-2180