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Table 1 Bacterial strains and plasmids used in this study

From: A previously uncharacterized gene stm0551 plays a repressive role in the regulation of type 1 fimbriae in Salmonella enterica serotype Typhimurium

Name Description a Reference or source
Salmonella enterica serotype Typhimurium
LB5010 Wild type S. enterica serotype Typhimurium LT2 strain, fimbriate with the complete fim gene cluster [21]
Δstm0551 stm0551 deletion mutant; Kanr Present study
Escherichia coli strain
One Shot® TOP10 chemically competent E. coli F-mcr A Δ(mrr-hsd RMS-mcr BC) Φ80lacZ ΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G Invitrogen
BL21Star™ (DE3) One Shot® chemically competent E. coli F-ompT hsdS B (rB- mB-) gal dcm (DE3) Invitrogen
Plasmids
pSTM0551 A 0.5-kb DNA fragment possessing the stm0551 coding sequence cloned into the pACYC184 vector; Cmr Present study
pSTM0551E49A A 0.5-kb DNA fragment possessing the stm0551 coding sequence with glutamic acid (E) at position 49 replaced with an alanine (A) cloned into the pACYC184 vector; Cmr Present study
pACYC184 Tetr, Cmr, cloning vector; w/p15A ori ATCC, Manassas, VA
pET101/D-TOPO Ampr, 6xHis tag expression vector Invitrogen
pKD46 Ampr, express λ Red recombinase system, temp- sensitive replicon [22]
pKD13 Plasmid carrying Kanr cassette [22]
  1. aAmpr ampicillin resistant; Cmr chloramphenicol resistant; Kanr kanamycin resistant; Tetr tetracycline resistant