Figure 6

Metabolites with different contents in cultures initiated with high or low spore densities. (A) A PLS scores plot, performed using SIMCA-P V11.0, for metabolites extracted from mycelia cultured for 2, 3, 4 and 5 days in PMS media with initial spore densities of 10⁴ (black) and 10⁶ (gray) spores/ml, with 3 replicates in each treatment. (B) Scatter loading plots obtained from PLS analyses of the entire GC-Tof-MS dataset. (C and D) Total ion chromatographies of metabolites extracted from mycelia of A. flavus grown in PMS media for 3 days with the initial spore densities of 10⁴ (C) and 10⁶ spores/ml (D). Metabolites with significant differences in quantity between (C) and (D) are labeled.