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Table 1 Bioinformatically determined putative adhesins in the O157 DMEM-Proteome

From: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

Protein Identity: Sequences not homologous [O-island] OR homologous [Backbone] toE. coliK12 MG1655

Peptide Hits

Bacterial Cell Localization

Proteins identified by PELS1

Proteins identified by IVIAT2

Proteins associated with O157 viulence

Tir: Translocated intimin receptor protein: O-island#148

3

Extracellular

  

+

Eae: Intimin: Adhesin; attaching and effacing protein: O-island #148

36

Outer Membrane

  

+

ChuA: Outer membrane heme/hemoglobin receptor; heme utilization/transport protein: O-island #140

13

Outer Membrane

 

+

+

EspP: Serine protease; secreted autotransporter: pO157

6

Outer Membrane

+

 

+

TerE: Putative tellurium resistance protein B (Putative phage inhibition, colicin resistance): O-island #43

35

Cytoplasm

   

Z2239: Putative outer membrane porin protein: O-island #62

2

Outer Membrane

   

FimG: Fimbrial Morphology: Backbone

1

Inner Membrane

   

MsrA: Peptide methionine sulfoxide reductase : Backbone

1

Cytoplasm

   

YjeI: Putative lipoprotein :Backbone

2

Non-cytoplasmic

   

BtuB: Outer membrane receptor for transport of vitamin B12, E colicins, and bacteriophage: Backbone

3

Outer Membrane

   

DsbA: Thiol:disulfide interchange protein precursor : Backbone

8

Periplasm

   

PstS: High-affinity phosphate-specific transport system; periplasmic phosphate-binding protein: Backbone

3

Periplasm

   

YiaF: Hypothetical protein: Backbone

2

Cytoplasm

   

Slp: Outer membrane protein induced after carbon starvation: Backbone

17

Outer Membrane

   

LivJ: Leu/Ile/Val-binding protein precursor; ABC transporter: Backbone

2

Periplasm

   

PpiA: Peptidyl-prolyl cis-trans isomerase A precursor : Backbone

4

Cytoplasm

   

YrbD: Hypothetical protein precursor; probable phospholipid ABC transporter-binding protein MlaD :Backbone

1

Periplasm

   

YraP: Hypothetical protein; putative transport :Backbone

2

Periplasm

   

TolC: Outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosome: Backbone

3

Outer Membrane

+

  

Lpp: Murein-lipoprotein; major outer membrane lipoprotein precursor: Backbone

31

Outer Membrane

+

MepA, MltB, Slt

 

NlpB: Lipoprotein-34: Backbone

3

Outer Membrane

   

Z3508: Hypothetical protein :Backbone

1

Non-cytoplasmic

   

OmpC: Outer membrane protein 1b: hyperosmotic shock: Backbone

123

Outer Membrane

+

  

YehZ: Putative transport system permease protein :Backbone

1

Cytoplasm

   

CspC: Cold shock-like protein : Backbone

25

Cytoplasm

   

CspD: Cold shock-like protein : Backbone

1

Cytoplasm

   

CspE: Cold shock-like protein : Backbone

9

Cytoplasm

   

YeaF: Hypothetical protein yeaF; putative scaffolding protein in the formation of a murein :Backbone

4

Outer Membrane

+

YeaA

 

OsmE: Osmotically inducible lipoprotein E precursor; activator of ntr-like gene : Backbone

9

Cytoplasm

   

SlyB: Outer membrane lipoprotein slyB precursor : Backbone

3

Outer Membrane

   

YciD: Putative outer membrane protein :Backbone

1

Outer Membrane

   

YceI: Putative GTP binding :Backbone

2

Non-cytoplasmic

   

ArtI: Arginine 3rd transport system periplasmic binding protein: Backbone

1

Periplasm

   

YbiS: Putative transpeptidase :Backbone

5

Non-cytoplasmic

   

OmpX: Outer membrane protein X precursor : Backbone

22

Outer Membrane

   

FepA: Receptor for ferric enterobactin (Enterochelin) and colicins B and D: Backbone

29

Outer Membrane

+

  
  1. 1PELS: Proteomics- based Expression Library Screening
  2. 2IVIAT:In Vivo-Induced Antigen Technology