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Figure 3 | BMC Microbiology

Figure 3

From: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

Figure 3

Adherence patterns of O157 strains on HEp-2 cells, in the presence of D + Mannose and +/− antisera. Panel A, O157 strain EDL933, in the presence of “pooled antisera” against LEE. Intimin and flagellar H7 proteins, and the anti-Intimin antisera alone, at 1:100 and 1:10 dilutions, respectively. Panel B, O157 strain EDL933, in the absence of any sera (No sera). Panel C, O157 strain 86–24 (Intimin-positive) and its mutant derivatives, 86-24eae Δ10 (Intimin-negative), and 86-24eae Δ10 (pEB310) (Initmin-positive) in the absence of any sera. The immunofluorescence (IF) stained slides are shown at 40x magnification. O157 have green fluorescence, actin filaments of HEp-2 cells have orange-red fluorescence, and their nuclei have blue fluorescence.

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BMC Microbiology

ISSN: 1471-2180

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