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Table 5 Bacterial strains and plasmids used in this study

From: Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

Strain or plasmid Relevant characteristicsa Reference or source
Escherichia coli   
   DH5α recA lacZΔM15 [27]
   CECT831 Indicator strain of mangotoxin production CECTb
Pseudomonas fluorescens   
   Pf-5 Complete genome sequenced and free access. [28]
Pseudomonas syringae pv. syringae  
   B728a Complete genome sequenced and free access [16]
   UMAF0158 Wild type isolated from mango, mangotoxin producer, Nfr [12]
UMAF0158-2βB7
UMAF0158-3αE10
miniTn5 mutants of UMAF0158 in gacA and gacS respectively, defective in mangotoxin, Kmr, Nfr [15]
UMAF0158-3νH1
UMAF0158-6νF6
miniTn5 mutants of UMAF0158, defective in mangotoxin production, Kmr, Nfr [15]
UMAF2-6-3H1
UMAF2-6A
miniTn5 mutants from UMAF0158 and complemented with plasmid pCG2-6, production of mangotoxin restored, Kmr, Ampr, Nfr This study
   [15]
   UMAF0158::ORF0c ORF0 mutant of UMAF0158, ORF0::pCR-ORF0, Kmr, Nfr This study
   UMAF0158::ORF1 ORF1 mutant of UMAF0158, ORF1::pCR::ORF1, Kmr, Nfr This study
   UMAF0158::ORF2 ORF2 mutant of UMAF0158, ORF2::pCR::ORF2, Kmr, Nfr This study
   UMAF0158::mgoB mgoB (ORF3) mutant of UMAF0158, mgoB::pCR::mgoB; Kmr, Nfr This study
   UMAF0158::mgoC mgoC (ORF4) mutant of UMAF0158, mgoC::pCR::mgoC; Kmr, Nfr This study
   UMAF0158::mgoA mgoA (ORF5) mutant of UMAF0158, mgoA::pCR::mgoA; Kmr, Nfr This study
   UMAF0158::mgoD mgoD (ORF6) mutant of UMAF0158, mgoD::pCR::mgoD; Kmr, Nfr This study
   UMAF0158ΔmgoA mgoA (ORF5) mutant of UMAF0158 by deletion, Nfr This study
Plasmids   
   pGEM-T Cloning vector, Ampr Invitrogen, California, USA
   pCR2.1 Cloning vector lacZ, Kmr, Ampr, Invitrogen, California, USA
   pBBR1MCS-5 Cloning vector, Gmr [29]
   pMP220 Promoter-probe vector containing a promoterless lacZ gene [30]
   pCR::ORF0 integrative plasmid pCR2.1 carrying ORF0 This study
   pCR::ORF1 integrative plasmid pCR2.1 carrying ORF1 This study
   pCR::ORF2 integrative plasmid pCR2.1 carrying ORF2 This study
   pCR::mgoB integrative plasmid pCR2.1 carrying mgoB This study
   pCR::mgoC integrative plasmid pCR2.1 carrying mgoC This study
   pCR::mgoA integrative plasmid pCR2.1 carrying mgoA This study
   pCR::mgoD integrative plasmid pCR2.1 carrying mgoD This study
   pCG2-6 genomic clone of UMAF0158 GenBank-DQ532441 [15]
   pLac36 From mgoB to mgoD cloned in pBBR1MCS-5 This study
   pLac56 mgoA and mgoD cloned in pBBR1MCS-5 This study
   pLac6 mgoD cloned in pBBR1MCS-5 This study
   pMPmgo pMP220 vector containing the putative promoters of mgo operon This study
   pEMG integrative plasmid for deletion mutagenesis, Kmr. [31]
   pSW-2 plasmid carrying I-SceI gene for deletion mutagenesis, Gmr. [31]
  1. a) Ampr: ampicillin resistance; Gmr: gentamycin resistance; Kmr: kanamycin resistance; Nfr: Nitrofurantoin resistance. b) CECT: Spanish Type Culture Collection. c) ORF0 was named in this way because it was cloned as an uncompleted ORF