Table 5 Bacterial strains and plasmids used in this study

Escherichia coli

   DH5α

recA lacZΔM15

[27]

   CECT831

Indicator strain of mangotoxin production

CECT^b

Pseudomonas fluorescens

   Pf-5

Complete genome sequenced and free access.

[28]

Pseudomonas syringae pv. syringae

   B728a

Complete genome sequenced and free access

[16]

   UMAF0158

Wild type isolated from mango, mangotoxin producer, Nf^r

[12]

UMAF0158-2βB7

UMAF0158-3αE10

miniTn5 mutants of UMAF0158 in gacA and gacS respectively, defective in mangotoxin, Km^r, Nf^r

[15]

UMAF0158-3νH1

UMAF0158-6νF6

miniTn5 mutants of UMAF0158, defective in mangotoxin production, Km^r, Nf^r

[15]

UMAF2-6-3H1

UMAF2-6A

miniTn5 mutants from UMAF0158 and complemented with plasmid pCG2-6, production of mangotoxin restored, Km^r, Amp^r, Nf^r

This study

[15]

   UMAF0158::ORF0^c

ORF0 mutant of UMAF0158, ORF0::pCR-ORF0, Km^r, Nf^r

This study

   UMAF0158::ORF1

ORF1 mutant of UMAF0158, ORF1::pCR::ORF1, Km^r, Nf^r

This study

   UMAF0158::ORF2

ORF2 mutant of UMAF0158, ORF2::pCR::ORF2, Km^r, Nf^r

This study

   UMAF0158::mgoB

mgoB (ORF3) mutant of UMAF0158, mgoB::pCR::mgoB; Km^r, Nf^r

This study

   UMAF0158::mgoC

mgoC (ORF4) mutant of UMAF0158, mgoC::pCR::mgoC; Km^r, Nf^r

This study

   UMAF0158::mgoA

mgoA (ORF5) mutant of UMAF0158, mgoA::pCR::mgoA; Km^r, Nf^r

This study

   UMAF0158::mgoD

mgoD (ORF6) mutant of UMAF0158, mgoD::pCR::mgoD; Km^r, Nf^r

This study

   UMAF0158ΔmgoA

mgoA (ORF5) mutant of UMAF0158 by deletion, Nf^r

This study

Plasmids

   pGEM-T

Cloning vector, Amp^r

Invitrogen, California, USA

   pCR2.1

Cloning vector lacZ, Km^r, Amp^r,

Invitrogen, California, USA

   pBBR1MCS-5

Cloning vector, Gm^r

[29]

   pMP220

Promoter-probe vector containing a promoterless lacZ gene

[30]

   pCR::ORF0

integrative plasmid pCR2.1 carrying ORF0

This study

   pCR::ORF1

integrative plasmid pCR2.1 carrying ORF1

This study

   pCR::ORF2

integrative plasmid pCR2.1 carrying ORF2

This study

   pCR::mgoB

integrative plasmid pCR2.1 carrying mgoB

This study

   pCR::mgoC

integrative plasmid pCR2.1 carrying mgoC

This study

   pCR::mgoA

integrative plasmid pCR2.1 carrying mgoA

This study

   pCR::mgoD

integrative plasmid pCR2.1 carrying mgoD

This study

   pCG2-6

genomic clone of UMAF0158 GenBank-DQ532441

[15]

   pLac36

From mgoB to mgoD cloned in pBBR1MCS-5

This study

   pLac56

mgoA and mgoD cloned in pBBR1MCS-5

This study

   pLac6

mgoD cloned in pBBR1MCS-5

This study

   pMPmgo

pMP220 vector containing the putative promoters of mgo operon

This study

   pEMG

integrative plasmid for deletion mutagenesis, Km^r.

[31]

   pSW-2

plasmid carrying I-SceI gene for deletion mutagenesis, Gm^r.

[31]