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Table 2 Specific inhibition using dilutions of cell-free culture filtrates from Pseudomonas syringae pv.syringae UMAF0158 and its derived miniTn5 and insertion mutants grown in liquid minimal medium (PMS).

From: Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

Bacterial strains Mangotoxin production Dilutions of cultures filtratesa
   1:1 1:2 1:4 1:8 + ornithine
Wild type       
UMAF0158 + 21.7 ± 0.4 18.2 ± 0.4 13.7 ± 0.4 9.5 ± 0.5 < 7
miniTn5 mutants       
UMAF0158-3νH1 - < 7 < 7 < 7 < 7 < 7
UMAF0158-6νF6 - < 7 < 7 < 7 < 7 < 7
pCG2-6 complementation      
UMAF2-6-3H1 + 19.0 ± 1.0 15.5 ± 0.5 13.5 ± 0.5 9.5 ± 0.5 < 7
UMAF2-6A + 19.0 ± 0.7 16.2 ± 0.4 12.7 ± 1.3 10.5 ± 0.5 < 7
Insertion mutants      
UMAF0158::ORF1 + 20.2 ± 1.3 17.0 ± 0.7 14.7 ± 0.8 11.0 ± 0.8 < 7
UMAF0158::ORF2 + 19.7 ± 1.5 16.2 ± 0.8 12.2 ± 1.1 < 7 < 7
UMAF0158::mgoB + 17.7 ± 0.8 14.2 ± 0.8 12.0 ± 0.8 < 7 < 7
UMAF0158::mgoC - < 7 < 7 < 7 < 7 < 7
UMAF0158::mgoA - < 7 < 7 < 7 < 7 < 7
UMAF0158::mgoD - < 7 < 7 < 7 < 7 < 7
pLac complementation     
UMAF0158-6νF6 containing pLac56 + 19.2 ± 0.4 15.7 ± 0.8 12.7 ± 1.2 < 7 < 7
UMAF0158-6νF6 containing pLac6 - < 7 < 7 < 7 < 7 < 7
  1. The inhibition analysis was performed by Escherichia coli growth inhibition test
  2. a) Toxic activity is expressed as diameter of inhibition zone (in mm). Average and standard deviation values were obtained from three replicate of three independent experiments