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Table 1 Characterization of disrupted genes surrounding the mgo operon in derivates miniTn5 and insertional mutants from the wild type Pseudomonas syringae pv.syringae UMAF0158 mangotoxin producer

From: Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

Bacterial strains ORF disrupted Mangotoxin productiona Putative homology of disrupted gene Comparison ncl-nclb with Pss B728a
     % of identity gene name
miniTn5 mutants c      
UMAF0158-3νH1 mgoC - Conserved hypothetical protein 95 Psyr_5010
UMAF0158-6νF6 mgoA - Nonribosomal peptide synthetase 93 Psyr_5011
Insertional mutants     
UMAF0158::ORF0 ORF0 + HAD hydrolase 92 Psyr_5006
UMAF0158::ORF1 ORF1 + Aldo-keto oxidoreductase 98 Psyr_5007
UMAF0158::ORF2 ORF2 + Transcriptional regulator GntR family 97 Psyr_5008
UMAF0158::mgoB mgoB (+) Haem-oxigenase-likee 96 Psyr_5009
UMAF0158::mgoC mgoC - p-aminobenzoate N-oxygenase AurFe 95 Psyr_5010
UMAF0158::mgoA mgoA - Nonribosomal peptide synthetase 93 Psyr_5011
UMAF0158::mgoD mgoD - Poliketide_cyc2d 94 Psyr_5012
  1. a) Presence of inhibition halo around the bacterial growth point in E. coli growth inhibition test. -: absence of inhibition halo, +: presence of inhibition halo, (+): slight mangotoxin production (smaller and opaque halo)
  2. b) ncl-ncl is used as abbreviation of nucleotide-nucleotide
  3. c) miniTn5 mutants defective in mangotoxin production obtained in a previous work [15]
  4. d) Putative function by family domains searches