Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

Table 1 Characterization of disrupted genes surrounding the mgo operon in derivates miniTn5 and insertional mutants from the wild type Pseudomonas syringae pv.syringae UMAF0158 mangotoxin producer

Bacterial strains	ORF disrupted	Mangotoxin production^a	Putative homology of disrupted gene	Comparison ncl-ncl^b with Pss B728a
				% of identity	gene name
miniTn5 mutants ^c
UMAF0158-3νH1	mgoC	-	Conserved hypothetical protein	95	Psyr_5010
UMAF0158-6νF6	mgoA	-	Nonribosomal peptide synthetase	93	Psyr_5011
Insertional mutants
UMAF0158::ORF0	ORF0	+	HAD hydrolase	92	Psyr_5006
UMAF0158::ORF1	ORF1	+	Aldo-keto oxidoreductase	98	Psyr_5007
UMAF0158::ORF2	ORF2	+	Transcriptional regulator GntR family	97	Psyr_5008
UMAF0158::mgoB	mgoB	(+)	Haem-oxigenase-like^e	96	Psyr_5009
UMAF0158::mgoC	mgoC	-	p-aminobenzoate N-oxygenase AurF^e	95	Psyr_5010
UMAF0158::mgoA	mgoA	-	Nonribosomal peptide synthetase	93	Psyr_5011
UMAF0158::mgoD	mgoD	-	Poliketide_cyc2^d	94	Psyr_5012

a) Presence of inhibition halo around the bacterial growth point in E. coli growth inhibition test. -: absence of inhibition halo, +: presence of inhibition halo, (+): slight mangotoxin production (smaller and opaque halo)
b) ncl-ncl is used as abbreviation of nucleotide-nucleotide
c) miniTn5 mutants defective in mangotoxin production obtained in a previous work [15]
d) Putative function by family domains searches

ISSN: 1471-2180