Figure 3

Localisation and analysis of the promoter in the mgo operon. A) The design of the 5' RACE experiment, including the upstream and downstream sequences of the mgoB gene. B) The results obtained from the 5' RACE experiment. Lane 1, amplification from the primer GSP1; lane 2, amplification from the primer GSP2; lane 3, amplification from the primer GSP3; lane L, loading buffer and HyperLadder I (Bioline), with the different sizes indicated. C) The 3'-end of ORF2, with the stop codon in bold type, and the 5'-end of mgoB, with the start codon also in bold type, are indicated. The nucleotide sequence (814 bp) located between these two ORFs was analysed. The two putative promoters found in this sequence by the in silico analysis are indicated by the locations of the respective -10 and -35 boxes (in red); moreover, the sequence of the alternative -35 and -10 boxes, which are more closely related to Pseudomonas promoters, are marked in blue. The start of the transcript is marked as nucleotide +1 (with black point under the nucleotide). The putative ribosomal binding site (RBS) of mgoB is also indicated.