Figure 1

Organisation of the DNA cloned into pCG2-6 and the locations of the insertional and mini Tn5 mutants used in this study. pCG2-6 contains an 11,103-bp insert of chromosomal DNA derived from Pseudomonas syringae pv. syringae UMAF0158 (GenBank accession number DQ532441). The site of insertion or miniTn5 within the UMAF0158-3γH1 and UMAF0158-6γF6 mutants (▼) [15] as well as the design of the insertional mutants (↑) generated in the current study are indicated. The predicted sites of the putative promoters (►) and transcriptional terminators (○) are indicated. The putative mgo operon involved in mangotoxin production by UMAF0158 is illustrated by grey arrows. Each gene studied in this study was given a specific name. The ORFs upstream of the mgo operon are illustrated by white arrows, and the 5S and 23S ribosomal RNAs are indicated by black arrows.