Molecular analysis of methanogenic archaea in the forestomach of the alpaca (Vicugna pacos)

BMC Microbiology

Table 3 Percentage (%) in 16S rRNA gene clone distribution by taxon or phylum between alpacas

Taxa	Alpaca 4	Alpaca 5	Alpaca 6	Alpaca 8	Alpaca 9	Combined
*Methanobacteriales*
*Methanobrevibacter*
*ruminantium* ¹	16.2	11.6	7.0	28.6	12.3	15.0
*millerae* ¹	57.5	32.7	62.7	27.5	57.0	47.3
*smithii* ¹	6.1	5.0	3.5	4.8	2.2	4.3
unassigned ²	12.8	34.7	15.4	30.7	10.6	21.1
*Methanobacterium*
unassigned ²	5.6	13.1	4.5	4.2	8.9	7.3
*Methanosphaera*
unassigned ²	1.7	1.5	0.5	3.2	5.6	2.4
*Thermoplasmatales*
unassigned ³	0.0	1.5	6.5	1.0	3.4	2.5

¹sequences in OTUs that have 98% or higher sequence identity to the 16S rRNA gene of the specified species
²sequences in OTUs that have 95-97.9% sequence identity to the16S rRNA gene of a valid species from the specified genera
³sequences in OTUs that have 80-83% sequence identity to the 16S rRNA gene of Aciduliprofundum boonei and are likely part of a new order of uncultured archaea

ISSN: 1471-2180