|
Escherichia coli
| | |
|
1830
|
pro¯ met¯ Kanr Nmr, containing transposon Tn5 on the suicidal plasmid pBJ4JI
|
[44]
|
|
DH5α
|
supE44ΔlacU169(Φ80lacZΔM15) hsdR17recA1 gyrA96thi-1relA1
|
[39]
|
|
BL21(DE3)
|
hsdS gal(λcIts857 ind1 Sam7 nin5 lac UV5-T7 gene 1)
|
[45]
|
|
Pectobacterium carotovorum
subsp.
carotovorum
| | |
|
3F-3
|
Pcc, wild-type
|
Laboratory stock
|
|
F-rif-18
|
3F3, Rifr, wild-type
|
This study
|
|
TF1-1
|
F-rif-18, fliC::Tn5, Rifr, Kanr
|
This study
|
|
TF 1-2
|
F-rif-18, CarocinS2::Tn5, Rifr, Kanr
|
This study
|
|
SP33
|
Pcc, wild-type
|
Laboratory stock
|
|
Plasmid
| | |
|
pMCL210
|
p15A, Cmlr, Low copy number
|
[46]
|
|
pGEM T-Easy
|
Ampr; lacZ cloning vector
|
Promega
|
|
pET32a
|
Ampr; expression vector with the N-terminal His-tag
|
Novagen
|
|
pET30b
|
Kanr; expression vector with the C-terminal His-tag
|
Novagen
|
|
pMS2KI
|
5.7-kb BamHI DNA fragment harboring carocin S2 gene from 3F3 genome, cloned into pMCL210
|
This study
|
|
pEN2K*
|
caroS2K subcloned into pET32a
|
This study
|
|
pES2KI
|
Derived from pEN2K; deleted series of Tag element in front of expressed caroS2K
|
This study
|
|
pEH2KI*
|
Derived from pES2KI; adding (His)6-Tag adjacent to caroS2I
|
This study
|
|
pGS2I
|
caroS2I and its putative promoter from pMS2KI, subcloned into pGEM T-easy
|
This study
|
|
pECS2I*
|
caroS2I subcloned into pET30b, but the expressed fusion CaroS2I has no activity
|
This study
|
|
pES2I
|
Derived form pECS2I, the (His)6-Tag element was deleted
|
This study
|
