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Table 1 Changes in the gene expression of C. neoformans H99 cells exposed to FLC

From: Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A

BROAD ID (CNAG_*****) C. n. gene name S. c. gene name Description Fold change
Ergosterol biosynthesis
04804 SRE1   Sterol regulatory element-binding protein 1 + 4.04
01737   ERG25 C-4 methyl sterol oxidase + 3.95
00854   ERG2 C-8 sterol isomerase + 3.47
02896   ERG13 Hydroxymethylglutaryl-CoA synthase + 3.03
06644   ERG5 C-22 sterol desaturase + 2.50
00040 ERG11 ERG11 Lanosterol 14 alpha-demethylase + 2.47
06829   ERG1 Squalene monooxygenase + 2.37
00519   ERG3 C-5 sterol desaturase + 2.21
01129   ERG7 Lanosterol synthase + 2.09
Transport
04632   FUR4 Uracil permease + 5.87
07448   DUR3 Urea transporter + 4.78
04758   MEP2/AMP2 Ammonium transporter + 3.78
06652   DAL5 Allantoate permease + 2.83
01742   AQY1 Water channel + 2.73
07902   CAN1 Amino acid transporter + 2.52
01960   YMR279C Efflux protein EncT + 2.47
06338   PDR15 ABC transporter PMR5 + 2.37
04898   ATR1 MFS transporter + 2.37
00284   YOR378W Efflux protein EncT + 2.36
00097   ITR1 ITR1 + 2.26
00895   ZRT1 Low-affinity zinc ion transporter + 2.20
04210   MPH2 Sugar transporter + 2.15
04617   OPT2 Small oligopeptide transporter + 2.11
05592   PMR1 Calcium-transporting ATPase + 2.06
01059   YBR241C Vacuolar membrane protein + 2.02
00904   AZR1 Aflatoxin efflux pump AFLT - 2.10
01769   AGC1 Mitochondrial inner membrane protein - 2.16
04142   FEN2 Tartrate transporter - 2.17
04567   TPO2 Drug transporter - 2.22
05387   HXT5 Galactose transporter - 2.28
02355   YEA4 UDP-N-acetylglucosamine transporter - 2.30
05994   FLR1 Multidrug transporter - 2.35
02733   STL1 Hexose transport-related protein - 2.46
03794   YBR287W Endoplasmic reticulum protein - 2.58
00815   SIT1 Siderochrome-iron (Ferrioxamine) uptake transporter - 2.92
01354   TNA1 Transporter - 3.39
02104 SFH5 SFH5 Phosphatidylinositol transfer protein SFH5 - 4.54
07695   UGA4 Gamma-aminobutyric acid transporter - 5.16
00749   YIL166C Transporter - 5.65
02083   ARN2 Siderochrome-iron transporter - 9.48
Cell wall maintenance
02217   CHS7 Chitin synthase 7 + 3.62
06336   BGL2 Glucan 1,3 beta-glucosidase protein + 2.61
03326   CHS2 Chitin synthase 2, CHS2 + 2.20
01239 CDA3 CDA2 Chitin deacetylase - 4.35
Capsule biosynthesis
03644 CAS3   CAS3p + 12.16
01489 CAS9 YJL218W Putative O-acetyl transferase - 3.84
Lipid and fatty acid metabolism
06085 PLB1 PLB1 Phospholipase B + 2.18
06623 MIOX   Myo-inositol oxygenase + 2.12
03128   ECM38 Lincomycin-condensing protein lmbA - 2.01
00424   PCT1 Choline-phosphate cytidylyltransferase - 2.02
05042   CAT2 Carnitine acetyltransferase - 2.10
02000   FOX2 Short-chain dehydrogenase - 2.95
00834   PSD2 Phosphatidylserine decarboxylase - 3.10
02968 PLC2   Phospholipase C-2 - 4.11
Cell stress
03400   GRE2 Oxidoreductase + 3.54
05256   CTA1 Catalase 2 + 2.81
02440   HSC82 Cation-transporting ATPase + 2.54
01750 HSP70 SSA1 Heat shock protein 70 + 2.48
06917 TSA3 PRX1 Thiol-specific antioxidant protein 3 + 2.09
03185   LOT6 Low temperature-responsive protein + 2.05
04622   SNG1 Response to drug-related protein - 2.17
00575   CTT1 Catalase - 2.21
01464 FHB1 YHB1 Flavo-haemoglobin - 2.32
Amino acid metabolism
02284   PDA1 Branched-chain alpha-keto acid dehydrogenase E1-alpha subunit + 2.42
04862   GLT1 Glutamate synthase (NADH) + 2.39
04017   MXR2 Protein-methionine-R-oxide reductase + 2.32
01231   CAR1 Arginase + 2.27
03828   ARO8 Aromatic amino acid aminotransferase I + 2.26
06540   ILV3 Dihydroxy-acid dehydratase + 2.18
00247   LYS9 Saccharopine dehydrogenase (NADP+, L-glutamate-forming) + 2.02
02270   MET2 Homoserine O-acetyltransferase - 2.11
01076   UGA1 4-aminobutyrate transaminase - 2.18
00237   LEU1 3-isopropylmalate dehydratase - 2.27
01264   LYS12 Isocitrate dehydrogenase - 2.31
00879   GDH2 Glutamate dehydrogenase - 2.33
04467   UGA2 Succinate-semialdehyde dehydrogenase (NAD(P)+) - 2.83
02851   GLY1 Threonine aldolase - 3.04
02049   PUT1 Proline dehydrogenase - 5.74
05602   PUT2 1-pyrroline-5-carboxylate dehydrogenase - 6.65
Carbohydrate metabolism
06374   MAE1 Malic enzyme + 6.04
02225 CELC EXG1 Cellulase + 3.99
02552   TKL1 Transketolase + 3.28
04025   TAL1 Transaldolase + 3.00
00696   AMS1 Alpha-mannosidase + 2.52
05913   MAL12 Alpha-glucosidase + 2.34
05113   ALD4 Aldehyde dehydrogenase (ALDDH) + 2.11
05264   YJL216C Alpha-amylase AmyA + 2.08
03946   GAL1 Galactokinase - 2.16
07752 GLF   UDP-galactopyranose mutase - 2.23
04659   PDC1 Pyruvate decarboxylase - 2.33
06924   SUC2 Beta-fructofuranosidase - 2.57
00269   SOR1 Sorbitol dehydrogenase - 2.62
00393 GLC3 GLC3 1,4-alpha-glucan-branching enzyme - 2.93
07745 MPD1 ADH3 Mannitol-1-phosphate dehydrogenase - 3.54
04217   PCK1 Phosphoenolpyruvate carboxykinase - 8.67
04621   GSY1 Glycogen (Starch) synthase - 11.00
04523   TDH3 Glyceraldehyde-3-phosphate dehydrogenase - 11.45
Protein biosynthesis, modification, transport, and degradation
02389   YPK1 AGC-group protein kinase + 3.04
02531   FUS3 Mitogen-activated protein kinase CPK1 + 2.91
03176   ERO1 Endoplasmic oxidoreductin 1 + 2.36
05932 CPR6 CPR6 Peptidyl-prolyl cis-trans isomerase D + 2.35
01861   NAS6 Proteolysis and peptidolysis-related protein + 2.35
04635   PEP4 Endopeptidase + 2.31
06872   YKL215C 5-oxoprolinase + 2.27
05005 ATG1 ATG1 Serine/threonine-protein kinase ATG1 + 2.20
00919   KEX1 Carboxypeptidase D + 2.13
04625   PRB1 Serine-type endopeptidase - 2.01
00130   RCK2 Serine/threonine-protein kinase - 2.12
04108   PKP1 Kinase - 2.17
02327   YFR006W Prolidase - 2.28
02418   DED81 Asparagine-tRNA ligase - 2.40
03563   DPS1 Aspartate-tRNA ligase - 2.50
04275   OMA1 Metalloendopeptidase - 2.50
02006   NTA1 Protein N-terminal asparagine amidohydrolase - 2.75
03949   PHO13 4-nitrophenylphosphatase - 3.32
TCA cycle
03596   KGD2 2-oxoglutarate metabolism-related protein - 2.02
03920   IDP1 Isocitrate dehydrogenase (NADP+) - 2.06
03674   KGD1 Oxoglutarate dehydrogenase (Succinyl-transferring) - 2.52
00747   LSC2 Succinate-CoA ligase (ADP-forming) - 2.70
07363   IDH2 Isocitrate dehydrogenase - 2.80
01137   ACO1 Aconitase - 2.99
07851   IDH1 Isocitrate dehydrogenase (NAD+), putative - 3.80
Glycerol metabolism
06132   RHR2 Glycerol-1-phosphatase + 2.31
02815   GUT2 Glycerol-3-phosphate dehydrogenase - 2.00
Nucleotide metabolism
05545   HNT2 Nucleoside-triphosphatase + 2.25
03078   NPP1 Type I phosphodiesterase/nucleotide pyrophosphatase family protein + 2.08
06489   ADO1 Adenosine kinase - 2.08
00613   FCY1 Cytosine deaminase - 2.69
Thiamin metabolism
03592   THI20 Phosphomethylpyrimidine kinase - 2.51
Alcohol metabolism
05258 SMG1   Glucose-methanol-choline (GMC) oxidoreductase + 6.67
05024   SPS19 L-xylulose reductase + 2.53
06168 GNO1 SFA1 GSNO reductase - 2.02
Carbon utilization
05144 CAN2 NCE103 Carbonic anhydrase 2 - 3.18
Cell cycle control     
03385   PCL1 G1/s-specific cyclin pcl1 (Cyclin hcs26) + 2.37
02604   HOP1 Putative uncharacterized protein + 2.19
00995   MSC1 Meiotic recombination-related protein - 3.63
Chromatin and chromosome structures
02115   NHP6B Nonhistone protein 6 - 2.47
Transcription
01841   GLN3 Predicted protein + 5.72
02990   YOR052C Nucleus protein + 2.16
04594   UGA3 PRO1 protein - 2.01
05290   SPT3 Transcription cofactor - 2.01
06495   RNH70 Ribonuclease H - 2.06
05333   PUT3 Putative uncharacterized protein - 2.14
02338   GIS2 DNA-binding protein hexbp - 2.47
05479   ASG1 Putative uncharacterized protein - 3.57
Signal transduction
03316   RDI1 Rho GDP-dissociation inhibitor 1 + 2.07
00363 HHK5 SLN1 CnHHK5 protein - 2.44
01262 GPB1 STE4 G-protein beta subunit GPB1 - 2.55
Oxidoreduction
04652   YLR460C Enoyl reductase + 2.63
06035   ADH1 Alcohol dehydrogenase + 2.41
00605   ZTA1 Cytoplasm protein + 2.20
00038   SOR2 Alcohol dehydrogenase + 2.13
01954   YPR127W Aldo/keto reductase + 2.09
02958   FET5 Ferroxidase + 2.06
02935   YMR226C Oxidoreductase - 2.01
01558   XYL2 Zinc-binding dehydrogenase - 2.28
00876   FRE7 Ferric-chelate reductase - 2.49
03168   MET10 Sulfite reductase (NADPH) - 2.55
07862   YEL047C Fumarate reductase (NADH) - 2.58
03498   FRE2 Metalloreductase - 2.85
03874   AIF1 Oxidoreductase - 2.89
Other
00331   YMR210W Anon-23da protein + 3.43
04934 TAR1   Temperature associated repressor + 2.37
05678   ADY2 Membrane protein + 2.28
00818   AGE2 AGD15 + 2.23
04867   YJR054W Vacuole protein + 2.22
06574 APP1   Antiphagocytic protein 1 + 2.21
06482   AMD2 Amidase + 2.20
01252   TUM1 Thiosulfate sulfurtransferase - 2.05
03452   AFG1 AFG1 family mitochondrial ATPase - 2.16
05831   MMF1 Brt1 - 2.19
03991   YGR149W Integral to membrane protein - 2.39
02039   YPL264C Integral membrane protein - 2.46
02943   SLM1 Cytoplasm protein - 2.49
06668   AIM38 Mitochondrion protein - 2.61
00638   LSG1 GTPase - 2.89
01653 CIG   Cytokine inducing-glycoprotein - 3.26
04314   YEF1 NAD+ kinase - 3.74
04690   FMP41 Mitochondrion protein - 5.52
  1. Genes that were found to be differentially expressed were ordered by expression level and categorized, if available, into functional groups as described in Materials and Methods. Results are presented as the mean fold-increase (symbol +) or -decrease (symbol -) of biological triplicates. Abbreviations: C. n., C. neoformans; S. c., S. cerevisiae.
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BMC Microbiology

ISSN: 1471-2180

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