Figure 4

Subcellular localization of YqiC. Whole-cell lysate of S. Typhimurium was fractionated by ultracentrifugation. Samples of the cell lysate (L), the supernatant (S) and the sedimented membrane fraction (M) were analyzed by immunoblotting with anti-YqiC and anti-MBP antiserum. Antibodies against the soluble MBP protein [10] was used as a control for the membrane fraction contamination.