Figure 6From: Integration Host Factor (IHF) binds to the promoter region of the phtD operon involved in phaseolotoxin synthesis in P. syringaepv. phaseolicola NPS3121Evaluation of the effect of mutations in the proposed IHF binding site. Gel mobility shift assays using the mutant probes of fragment I (104 bp). Panel A shows the assays using mutant probe 1, which contains changes in the dA-dT rich upstream region as well as changes of C to A and G to T in the consensus sequence. These changes caused a decrease of 89% with respect to the control. Panel B shows assays using mutant probe 2, which also includes mutations in the TTR region of the consensus sequence, causing an 86% decrease in the retarded signal. The asterisks indicate the bases modified. The bold red letters indicate the proposed site for IHF binding.Back to article page