|
LS107
|
syn-1000, bla-6, amps derivative of NA1000
|
Stephens et al. [45]
|
|
JG1172
|
syn-1000 bla-6 ΔfliX
|
Muir et al. [38]
|
|
SC1032
|
flbD198::Tn5
|
Ohta et al. [41]
|
|
E. coli
|
|
S17-1
|
Rp4-2, Tc::Mu, Km::Tn7
|
Simon et al. [46]
|
|
BL21(DE3)
|
F-ompT gal [dcm] [lon] hsdS
B
(rB- mB-; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene
|
Novagen
|
|
Plasmids
|
|
pX21b
|
derivative of pET-21b carrying histidine-tagged FliX under the control of T7 promoter, Apr
|
Muir & Gober [36]
|
|
pBBR1MCS
|
broad host range cloning vector, multicopy, Cmr
|
Kovach et al. [47]
|
|
pZXfliX
|
derivative of pBBR1MCS carrying PCR generated 894 bp BamHI-HindIII fragment containing fliX gene and its promoter
|
This study
|
|
pZX71A
|
derivative of pZXfliX, codon 71 cgcc→gcc
|
This study
|
|
pZX85K
|
derivative of pZXfliX, codon 85 ctg→aag
|
This study
|
|
pZXΔ117-8
|
derivative of pZXfliX, deletion of codon 117 and 118
|
This study
|
|
pZX130L
|
derivative of pZXfliX, codon 130 acc→ctg
|
This study
|
|
pZX136K
|
derivative of pZXfliX, codon 136 ctg→aag
|
This study
|
|
pfliX1
|
derivative of pBBR1-MCS expressing fliX 1, an allele of fliX carrying a point mutation at the sixteenth codon (R16G), a frame shift at codon 141, and an extended carboxyl terminus of 67 amino acids
|
Muir et al. [38]
|
|
pfliF/lacZ/290
|
fliF-lacZ transcriptional reporter vector, Tcr
|
Wingrove & Gober [48]
|
|
pfliK/lacZ/290
|
fliK-lacZ transcriptional reporter vector, Tcr
|
Gober & Shapiro [25]
|
