Figure 3
From: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
The cHtrA but not other chlamydial periplasmic proteins are secreted into host cell cytosol. HeLa cells infected with C. trachomatis organisms were processed and co-labeled with mouse antibodies against various periplasmic proteins (red) and a rabbit antibody against IncA (green) as described in Figure 1 legend. The Hoechst dye was used to visualize DNA (blue). The triple labeling was analyzed under a conventional fluorescence microscope (A) and confocal microscope (B). Under the confocal microscope, a series of four images were taken along the Z-axis by varying 1 μM between each. Note that cHtrA (red arrows) but none of the other periplasmic proteins including CT067, CT539 & CT783 was detected outside of the inclusion membrane (green arrows) by either immunofluorescence microscopy or confocal microscopy.