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Table 2 List of plasmids used in this study

From: The analysis of para-cresol production and tolerance in Clostridium difficile 027 and 012 strains

Plasmid Relevant properties Source
pGEM®-T Easy Commercial TA' cloning plasmid Promega
pMTL007 ClosTron mutagenesis plasmid Heap et al. 2007
pLDhpdB pMTL007 carrying Ll.LtrB intron retargetted to hpdB This work
pLDhpdC1 pGEM®-T Easy carrying Ll.LtrB intron retargetted to hpdC This work
pLDhpdC2 pMTL007 carrying Ll.LtrB intron retargetted to hpdC This work
pLDhpdA1 pGEM®-T Easy carrying Ll.LtrB intron retargetted to hpdA This work
pLDhpdA2 pMTL007 carrying Ll.LtrB intron retargetted to hpdA This work